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Literature DB >> 6146986

A new preparation for recording single-channel currents from skeletal muscle.

N B Standen, P R Stanfield, T A Ward, S W Wilson.

Abstract

Spherical vesicles of sarcolemma (media diameter, 78 microns) were prepared from frog skeletal muscle by prolonged exposure to enzymes (collagenase, then protease) and to isotonic KCl solutions, which promote swelling of muscle fibres. Patch clamp recording was used to measure unitary K and Na currents from this preparation. Na and K channels had conductances around 15 pS and their kinetic properties appeared unaltered by vesicle formation.

Entities: Chemical

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Year: 1984 PMID： 6146986 DOI： 10.1098/rspb.1984.0044

Source DB: PubMed Journal: Proc R Soc Lond B Biol Sci ISSN： 0950-1193

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Cited

23 in total

1. The beta1a subunit regulates the functional properties of adult frog and mouse L-type Ca2+ channels of skeletal muscle.

Authors: Rubén García; Elba Carrillo; Santiago Rebolledo; María C García; Jorge A Sánchez
Journal: J Physiol Date: 2002-12-01 Impact factor: 5.182

2. A macro cell-attached patch-clamp study of the properties of the Na current in the vicinity of the motor endplate region of frog single interosseal skeletal muscle fibres.

Authors: C O Malécot; A Duval
Journal: Pflugers Arch Date: 1992-03 Impact factor: 3.657

3. The effect of intracellular pH on ATP-dependent potassium channels of frog skeletal muscle.

Authors: N W Davies; N B Standen; P R Stanfield
Journal: J Physiol Date: 1992-01 Impact factor: 5.182

4. Activation of ATP-sensitive K channels by a K channel opener (SR 44866) and the effect upon electrical and mechanical activity of frog skeletal muscle.

Authors: M P Sauviat; E Ecault; J F Faivre; I Findlay
Journal: Pflugers Arch Date: 1991-04 Impact factor: 3.657

A new preparation for recording single-channel currents from skeletal muscle.

1. The beta1a subunit regulates the functional properties of adult frog and mouse L-type Ca2+ channels of skeletal muscle.

2. A macro cell-attached patch-clamp study of the properties of the Na current in the vicinity of the motor endplate region of frog single interosseal skeletal muscle fibres.

3. The effect of intracellular pH on ATP-dependent potassium channels of frog skeletal muscle.

4. Activation of ATP-sensitive K channels by a K channel opener (SR 44866) and the effect upon electrical and mechanical activity of frog skeletal muscle.

5. Three types of single voltage-dependent potassium channels in the sarcolemma of frog skeletal muscle.

6. Properties of single potassium channels in vesicles formed from the sarcolemma of frog skeletal muscle.

7. Tensile strength and dilatational elasticity of giant sarcolemmal vesicles shed from rabbit muscle.

8. Transient outward K+ channels in vesicles derived from frog skeletal muscle plasma membranes.

9. The voltage-dependent block of ATP-sensitive potassium channels of frog skeletal muscle by caesium and barium ions.

10. The giant cardiac membrane patch method: stimulation of outward Na(+)-Ca2+ exchange current by MgATP.