Effects of Aroclor 1254 treatment on the in vitro hepatic metabolism of toluene, aniline and aminopyrine in hybrid sunfish.

The in vitro metabolism of the volatile aromatic hydrocarbon toluene by enzymes associated with the 12,000 g supernatant fraction of hybrid sunfish (Lepomis macrochirus X L. cyanellus X L. gibbosus) liver homogenates was studied. Aminopyrine demethylase (APDM) and aniline hydroxylase (AH) activities were measured. Intramuscular injections of Aroclor 1254 (a polychlorinated biphenyl) produced significant increases in APDM and AH activities (P less than or equal to 0.1, ANOVA). There were no significant changes in the metabolism of toluene, liver wet weights, or liver protein concentrations following treatment.