Some aspects of sodium efflux from single barnacle muscle fibres.

This mini-review attempts to summarize information about the efflux of 22Na from single barnacle muscle fibres, based on the use of the microinjection technique. The view is put forward that the Na efflux consists of three components: an ouabain-sensitive component, an ouabain-insensitive component (representing secondary active transport), and an Na-Na exchange diffusion component. Evidence is brought forward which supports the view that the ouabain-insensitive Na efflux is divisible operationally into 3 phases: (i) the cyclic nucleotide-sensitive phase, (ii) the Cai-sensitive phase, and (iii) the pHe-sensitive phase. It is shown how the barnacle muscle fibre preparation has yielded information about the validity of the cAMP-protein kinase hypothesis and how it can be used to shed some light on the post-translational mechanism of aldosterone action.