Literature DB >> 6135509

Immunocytochemical characterization of clathrin-associated proteins (CAPs). I. Neuronal distribution of CAPs, a component of clathrin-coated vesicles.

S Puszkin, A Andrés, C Ores, M P Lisanti, W J Schook.   

Abstract

Clathrin-associated proteins (CAPs) elicited antibodies in rabbits that were affinity-purified using CAPs-conjugated CNBr-Sepharose 4B. Anti-CAPs IgG formed immunoprecipitates with CAPs and with the clathrin-CAPs complex. Indirect immunoperoxidase-labeling in sections of rat brain cortex using anti-CAPs and anti-clathrin IgG yielded similar staining patterns. Coated perinuclear cisternae and coated vesicles became stained and easily distinguished. Intense staining also was found in synaptic boutons with label between most synaptic vesicles and as a thick crust surrounding coated vesicles. The data demonstrate that clathrin and CAPs polypeptides are in identical subcellular locations.

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Year:  1983        PMID： 6135509     DOI： 10.1007/bf00218108

Source DB:  PubMed          Journal:  Cell Tissue Res        ISSN： 0302-766X            Impact factor:   5.249


  18 in total

1.  Coated vesicles from pig brain: purification and biochemical characterization.

Authors:  B M Pearse
Journal:  J Mol Biol       Date:  1975-09-05       Impact factor: 5.469

2.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

3.  Brain clathrin: studies of its ultrastructural assemblies.

Authors:  M P Lisanti; W Schook; N Moskowitz; K Beckenstein; W S Bloom; C Ores; S Puszkin
Journal:  Eur J Biochem       Date:  1982-01

4.  Assembly units of clathrin coats.

Authors:  E Ungewickell; D Branton
Journal:  Nature       Date:  1981-01-29       Impact factor: 49.962

5.  Brain clathrin complex: II. Immunofluorescent correlation and biochemical affinity for actin.

Authors:  S Puszkin; M Lisanti; K Haver; E L Hua; N Moskowitz; W S Bloom; W J Schook
Journal:  J Histochem Cytochem       Date:  1982-06       Impact factor: 2.479

6.  Brain clathrin and clathrin-associated proteins.

Authors:  M P Lisanti; W Schook; N Moskowitz; C Ores; S Puszkin
Journal:  Biochem J       Date:  1982-02-01       Impact factor: 3.857

7.  Brain clathrin: immunofluorescent patterns in cultured cells and tissues.

Authors:  W S Bloom; K L Fields; S H Yen; K Haver; W Schook; S Puszkin
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

8.  Mechanochemical properties of brain clathrin: interactions with actin and alpha-actinin and polymerization into basketlike structures or filaments.

Authors:  W Schook; S Puszkin; W Bloom; C Ores; S Kochwa
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

9.  Turnover of transmitter and synaptic vesicles at the frog neuromuscular junction.

Authors:  B Ceccarelli; W P Hurlbut; A Mauro
Journal:  J Cell Biol       Date:  1973-05       Impact factor: 10.539

10.  Immunocytochemical localization of coated vesicle protein in rodent nervous system.

Authors:  T P Cheng; F I Byrd; J N Whitaker; J G Wood
Journal:  J Cell Biol       Date:  1980-08       Impact factor: 10.539
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  5 in total

Review 1.  The synaptic vesicle and the cytoskeleton.

Authors:  J H Walker; D V Agoston
Journal:  Biochem J       Date:  1987-10-15       Impact factor: 3.857

2.  Immunocytochemical characterization of clathrin-associated proteins (CAPs). II. Immunolocalization of CAPs using selectively-absorbed IgG solutions.

Authors:  M P Lisanti; A Andrés; A C Puszkin; C Ores; W J Schook; S Puszkin
Journal:  Cell Tissue Res       Date:  1983       Impact factor: 5.249

Review 3.  Neuronal protein NP185 is developmentally regulated, initially expressed during synaptogenesis, and localized in synaptic terminals.

Authors:  S Puszkin; D Perry; S Li; V Hanson
Journal:  Mol Neurobiol       Date:  1992 Summer-Fall       Impact factor: 5.590

Review 4.  Neuronal compartments and axonal transport of synapsin I.

Authors:  P Paggi; T C Petrucci
Journal:  Mol Neurobiol       Date:  1992 Summer-Fall       Impact factor: 5.590

5.  Presence of an extensive clathrin coat on the apical plasmalemma of the rat kidney proximal tubule cell.

Authors:  J S Rodman; D Kerjaschki; E Merisko; M G Farquhar
Journal:  J Cell Biol       Date:  1984-05       Impact factor: 10.539
  5 in total

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