Literature DB >> 6134451

A labour-saving method for the in vitro culture of Plasmodium falciparum.

R Reber-Liske.   

Abstract

The in vitro cultivation of Plasmodium falciparum requires at least daily changes of medium (Trager and Jensen, 1976). Addition of 50 mg per litre of hypoxanthine to medium RPMI 1640 permits to postpone the change of medium for up to 72 hours. A single subculture step is required to remove unlabelled hypoxanthine prior to the use of the cultured material in 3H-hypoxanthine incorporation assays. The method constitutes a considerable saving of time and medium.

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Year:  1983        PMID： 6134451

Source DB:  PubMed          Journal:  Acta Trop        ISSN： 0001-706X            Impact factor:   3.112


  4 in total

1.  Use of radioactive ethanolamine incorporation into phospholipids to assess in vitro antimalarial activity by the semiautomated microdilution technique.

Authors:  N Elabbadi; M L Ancelin; H J Vial
Journal:  Antimicrob Agents Chemother       Date:  1992-01       Impact factor: 5.191

2.  P. falciparum infected erythrocytes are capable of endocytosis.

Authors:  E R Burns; S Pollack
Journal:  In Vitro Cell Dev Biol       Date:  1988-05

3.  Maintenance of in vitro cultures of Babesia divergens and Babesia major at low temperatures.

Authors:  J Konrad; E U Canning; L P Phipps; J Donnelly
Journal:  Z Parasitenkd       Date:  1985

4.  Yeast-based high-throughput screen identifies Plasmodium falciparum equilibrative nucleoside transporter 1 inhibitors that kill malaria parasites.

Authors:  I J Frame; Roman Deniskin; Alison Rinderspacher; Francine Katz; Shi-Xian Deng; Robyn D Moir; Sophie H Adjalley; Olivia Coburn-Flynn; David A Fidock; Ian M Willis; Donald W Landry; Myles H Akabas
Journal:  ACS Chem Biol       Date:  2015-01-30       Impact factor: 5.100
  4 in total

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