Literature DB >> 6134227

Cloning and expression in Escherichia coli of a DNA fragment from Bacillus sphaericus coding for biocidal activity against mosquito larvae.

S Ganesan, H Kamdar, K Jayaraman, J Szulmajster.   

Abstract

A 3.7 kb DNA fragment from Bacillus sphaericus 1593 was cloned and expressed in E. coli using the plasmid pHV33. The level of larvicidal activity of the hybrid plasmid, pGsp03, against two species of mosquito larvae was comparable to that found with sporulating cell suspensions of B. sphaericus. A limited restriction cleavage map of the cloned insert is given.

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Year:  1983        PMID： 6134227     DOI： 10.1007/bf00326076

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  10 in total

1.  [A new variety of Bacillus thuringinesis very toxic to mosquitoes: B. thuringiensis var. israelensis serotype 14].

Authors:  H de Barjac
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1978-03-13

2.  Size of DNA determined by viscoelastic measurements: results on bacteriophages, Bacillus subtilis and Escherichia coli.

Authors:  L C Klotz; B H Zimm
Journal:  J Mol Biol       Date:  1972-12-30       Impact factor: 5.469

3.  Electrophoretic separation of Bacillus subtilis genes.

Authors:  R M Harris-Warrick; Y Elkana; S D Ehrlich; J Lederberg
Journal:  Proc Natl Acad Sci U S A       Date:  1975-06       Impact factor: 11.205

4.  A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis.

Authors:  R D Klein; E Selsing; R D Wells
Journal:  Plasmid       Date:  1980-01       Impact factor: 3.466

5.  Cloning and expression of Bacillus subtilis spore genes.

Authors:  C Bonamy; J Szulmajster
Journal:  Mol Gen Genet       Date:  1982

6.  Isolation of plasmid deletion Mutants and study of their instability.

Authors:  S B Primrose; S D Ehrlich
Journal:  Plasmid       Date:  1981-09       Impact factor: 3.466

7.  Insecticidal activity of recent bacterial isolates and their toxins against mosquito larvae.

Authors:  S Singer
Journal:  Nature       Date:  1973-07-13       Impact factor: 49.962

8.  Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli.

Authors:  H E Schnepf; H R Whiteley
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

9.  Comparative biochemistry of entomocidal parasporal crystals of selected Bacillus thuringiensis strains.

Authors:  D J Tyrell; L A Bulla; R E Andrews; K J Kramer; L I Davidson; P Nordin
Journal:  J Bacteriol       Date:  1981-02       Impact factor: 3.490

10.  Cloning and expression of the crystal protein genes from Bacillus thuringiensis strain berliner 1715.

Authors:  A Klier; F Fargette; J Ribier; G Rapoport
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
  10 in total
  6 in total

Review 1.  Bacillus thuringiensis and related insect pathogens.

Authors:  A I Aronson; W Beckman; P Dunn
Journal:  Microbiol Rev       Date:  1986-03

2.  Cloning of the gene for the larvicidal toxin of Bacillus sphaericus 2362: evidence for a family of related sequences.

Authors:  P Baumann; L Baumann; R D Bowditch; A H Broadwell
Journal:  J Bacteriol       Date:  1987-09       Impact factor: 3.490

3.  Toxicity of Bacillus sphaericus crystal toxin to adult mosquitoes.

Authors:  J E Stray; M J Klowden; R E Hurlbert
Journal:  Appl Environ Microbiol       Date:  1988-09       Impact factor: 4.792

4.  Expression of the larvicidal gene of Bacillus sphaericus 1593M in the cyanobacterium Anacystis nidulans R2.

Authors:  N Tandeau de Marsac; F de la Torre; J Szulmajster
Journal:  Mol Gen Genet       Date:  1987-09

Review 5.  Bacillus sphaericus as a mosquito pathogen: properties of the organism and its toxins.

Authors:  P Baumann; M A Clark; L Baumann; A H Broadwell
Journal:  Microbiol Rev       Date:  1991-09

6.  Biocide gene(s) and biocidal activity in different strains of Bacillus sphaericus. Expression of the gene(s) in E. coli maxicells.

Authors:  J Louis; K Jayaraman; J Szulmajster
Journal:  Mol Gen Genet       Date:  1984
  6 in total

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