Literature DB >> 6131257

A dot hybridisation assay for detection of rotavirus.

J Flores, E Boeggeman, R H Purcell, M Sereno, I Perez, L White, R G Wyatt, R M Chanock, A Z Kapikian.   

Abstract

A "dot" hybridisation technique for the detection of rotavirus in stools and other biological materials is described. The assay is based on the in-situ hybridisation of labelled single-stranded RNA probes, obtained by in-vitro transcription of rotavirus particles, to heat-denatured rotavirus RNA immobilised on nitrocellulose membranes. The method is highly specific and allows for the detection of as little as 8 pg of viral RNA. Its use for the detection of rotavirus in stool suspensions and rectal swabs obtained from children with diarrhoea may facilitate epidemiological studies of rotavirus gastroenteritis.

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Year:  1983        PMID： 6131257     DOI： 10.1016/s0140-6736(83)92811-8

Source DB:  PubMed          Journal:  Lancet        ISSN： 0140-6736            Impact factor:   79.321


  26 in total

1.  Rotavirus detection by dot blot hybridization assay using a non-radioactive synthetic oligodeoxynucleotide probe.

Authors:  J Fernández; A Sandino; A Yudelevich; L F Avendaño; A Venegas; V Hinrichsen; E Spencer
Journal:  Epidemiol Infect       Date:  1992-02       Impact factor: 2.451

2.  Detection of hepatitis A virus by extraction of viral RNA and molecular hybridization.

Authors:  J R Ticehurst; S M Feinstone; T Chestnut; N C Tassopoulos; H Popper; R H Purcell
Journal:  J Clin Microbiol       Date:  1987-10       Impact factor: 5.948

3.  DNA Probe for Lactobacillus delbrueckii.

Authors:  M Delley; B Mollet; H Hottinger
Journal:  Appl Environ Microbiol       Date:  1990-06       Impact factor: 4.792

4.  Dot hybridization assay for distinction of rotavirus serotypes.

Authors:  J Flores; K Y Green; D Garcia; J Sears; I Perez-Schael; L F Avendaño; W B Rodriguez; K Taniguchi; S Urasawa; A Z Kapikian
Journal:  J Clin Microbiol       Date:  1989-01       Impact factor: 5.948

5.  Specificity of dot hybridization assay in the presence of rRNA for detection of rotaviruses in clinical specimens.

Authors:  J Eiden; S Sato; R Yolken
Journal:  J Clin Microbiol       Date:  1987-09       Impact factor: 5.948

6.  Diagnosis of rotavirus infection with cloned cDNA copies of viral genome segments.

Authors:  M Lin; M Imai; A R Bellamy; N Ikegami; Y Furuichi; D Summers; D L Nuss; R Deibel
Journal:  J Virol       Date:  1985-08       Impact factor: 5.103

7.  Use of nucleic acid probes in the diagnosis of diarrheal disorders.

Authors:  P Echeverria; D N Taylor; J Seriwatana; O Sethabutr; A Chatkaeomorakot
Journal:  Indian J Pediatr       Date:  1987 Jan-Feb       Impact factor: 1.967

Review 8.  Human viral gastroenteritis.

Authors:  G Cukor; N R Blacklow
Journal:  Microbiol Rev       Date:  1984-06

9.  Detection of enteroviruses by spot hybridization.

Authors:  T Hyypiä; P Stålhandske; R Vainionpää; U Pettersson
Journal:  J Clin Microbiol       Date:  1984-03       Impact factor: 5.948

10.  Detection of adenovirus in nasopharyngeal specimens by radioactive and nonradioactive DNA probes.

Authors:  T Hyypiä
Journal:  J Clin Microbiol       Date:  1985-05       Impact factor: 5.948
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