Literature DB >> 6129116

Flow sorting of mouse pancreatic B cells by forward and orthogonal light scattering.

O Nielsen, J K Larsen, I J Christensen, A Lernmark.   

Abstract

Mouse islet cell suspensions were separated into subpopulations based on forward low angle and orthogonal scattered light. Separation fractions were analyzed for hormone content by radioimmunoassays. The pancreatic B-cells had the highest orthogonal light scattering, while they overlapped in forward low angle light scattering with other endocrine (glucagon, pancreatic polypeptide and somatostatin containing cells) and nonendocrine cells. Living and paraformaldehyde fixed islet cell suspensions showed similar distributions of combined light scattering.

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Year:  1982        PMID： 6129116     DOI： 10.1002/cyto.990030307

Source DB:  PubMed          Journal:  Cytometry        ISSN： 0196-4763


  4 in total

Review 1.  The biosociology of pancreatic B cells.

Authors:  D Pipeleers
Journal:  Diabetologia       Date:  1987-05       Impact factor: 10.122

2.  Pancreatic beta-cell purification by altering FAD and NAD(P)H metabolism.

Authors:  M J Smelt; M M Faas; B J de Haan; P de Vos
Journal:  Exp Diabetes Res       Date:  2008

3.  A method for high-throughput functional imaging of single cells within heterogeneous cell preparations.

Authors:  Adam S Neal; Austin M Rountree; Jared R Radtke; Jianzhu Yin; Michael W Schwartz; Christiane S Hampe; Jonathan D Posner; Vincenzo Cirulli; Ian R Sweet
Journal:  Sci Rep       Date:  2016-12-16       Impact factor: 4.379

4.  Dynamic changes in pancreatic endocrine cell abundance, distribution, and function in antigen-induced and spontaneous autoimmune diabetes.

Authors:  Klaus Pechhold; Xiaolong Zhu; Victor S Harrison; Janet Lee; Sagarika Chakrabarty; Kerstin Koczwara; Oksana Gavrilova; David M Harlan
Journal:  Diabetes       Date:  2009-02-19       Impact factor: 9.461
  4 in total

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