The monoamine-containing neurons in the brain of the garfish, Lepisosteus osseus.

The morphological organization of monoamine (MA)-containing neurons in the brain of the longnose gar (Lepisosteus osseus) was studied by means of fluorescence histochemical methods. In this species, one of the few living representatives of the holostean group of actinopterygian fishes, by far the largest number of MA cells is found within the preoptico-hypothalamic complex. Multitudinous small-sized MA cells, with a short club-like process protruding into the third ventricle, are present along the ependymal wall of the lateral and posterior hypothalamic recesses. This population of CSF-contacting MA cells, comprising both catecholamine (CA) and serotonin (5-HT) type cells, gives rise to numerous efferent fibers, some of which proceed rostrally toward the telencephalon while others course dorsally to reach the midbrain tegmentum and optic tectum. Many fibers, however, arborize directly within the hypothalamus, particularly around blood vessels where they form patches of highly fluorescent material. Small CA cells are also scattered along the preoptic recess wall. They do not directly contact the CSF but also appear to contribute to the CA innervation of telencephalon. At brain stem levels, a few CA cells are scattered at the base of the rostral midbrain, in the isthmal tegmentum, and in the central and dorsal (vagal lobe) portions of the medulla. Some CSF-contacting CA cells are also present around the central canal at upper spinal cord levels. One of the most striking features of the MA systems in Lepisosteus is the remarkable development of the 5-HT neuronal network. A prominent 5-HT cell column extends rostrocaudally in the raphe region from the caudal midbrain to upper spinal cord levels. In the caudal midbrain and isthmus, the 5-HT, cells also invade the lateral tegmentum and profusely innervate various brain stem structures as well as large portions of telencephalon, particularly the dorsal nucleus of area ventralis (Vd). The CA innervation of telencephalon is relatively weak, except in the olfactory bulb where numerous CA varicosities were found. These findings in Lepisosteus suggest that the pattern of MA system organization in the holostean brain is far more similar to that seen in primitive vertebrates, such as cyclostomes--where the 5-HT systems are highly elaborated relative to the CA systems--than it is to the pattern in more advanced fishes such as teleosts.