Electrophysiological analysis of rat renal sugar and amino acid transport. V. Acidic amino acids.

We have used electrophysiological techniques to study various aspects of the transport of glutamate and aspartate in proximal tubules of the rat kidney in vivo. Single tubular cells were punctured with microelectrodes and the response of the cell membrane potential to sudden luminal or peritubular applications of these amino acids was measured. The experiments indicated that a specific transport system exists for L-glutamate and L-aspartate in the brushborder membrane, which does not transport neutral or basic amino acids. The uptake of both L-amino acids from the lumen into the cell was found to be rheogenic, probably reflecting the cotransport of two Na+ ions together with one amino acid molecule. The transport system has a slightly greater affinity for L-glutamate, but transports the smaller L-aspartate somewhat faster. Besides the L-isomers also D-glutamate and D-aspartate were found to depolarize the tubular cells which suggests that also the D-isomers are absorbed in the tubule, however they do not seem to use the same transport system as the L-isomers. In addition to the transport system in the brushborder, a similar Na+-dependent, rheogenic transport system for L-glutamate and L-aspartate was also found in the peritubular cell membrane, as deduced from cell cell depolarizations in response to these substrates applied peritubularly. The simultaneous presence of Na-driven transport systems in the apical and basal cell membrane which is not found with other amino acids, may explain the high intracellular accumulation of L-glutamate and L-aspartate in the kidney and provides a rational basis for explaining clinically observed cases of dicarboxylic aminoacidurias.