Characteristics of monoamine oxidase activity in brain and other organs of the adult bullfrog, Rana catesbeiana.

1. Monoamine oxidase (MAO) activity was measured in brain, liver, kidney and intestine of the adult bullfrog by a fluorometric method. 2. All tissues contained both type A and type B MAO, on the basis of responses to specific inhibitors, but with different ratios in each tissue. 3. MAO activity was optimum at 30 degrees C. However, MAO type B showed greater activity changes related to incubation temperature than did type A. 4. The Michaelis constant (Km) of MAO also varied with temperature, with a nadir around 20 degrees C. The functional significance of this is not clear. 5. Arrhenius plots showed that the activation energy for MAO B was higher than for MAO A.