Literature DB >> 6120854

A simple method for the preparation of antibodies to the mitochondrial biotin-dependent carboxylases.

C McKeon, B Wolf.   

Abstract

Heterologous antiserum to the 3 biotin-dependent carboxylases was prepared by selective removal of these enzymes from human liver on an avidin-sepharose column. A carboxylase-avidin-sepharose matrix was used as an antigen to produce anti-carboxylase antibodies. The resultant antisera can be used to purify the specific carboxylases, to prepare monoclonal antibodies to these enzymes or to study inherited carboxylase deficiencies and biotin-dependent intermediary metabolism.

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Year:  1982        PMID： 6120854     DOI： 10.1007/bf01945114

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  12 in total

1.  Tissue fractionation studies. 12. Intracellular distribution of some dehydrogenases, alkaline deoxyribonuclease and iron in rat-liver tissue.

Authors:  H BEAUFAY; D S BENDALL; P BAUDHUIN; C DE DUVE
Journal:  Biochem J       Date:  1959-12       Impact factor: 3.857

2.  The binding of biotin to sepharose-avidin column. Demonstration of the affinity chromatography technique.

Authors:  A D Landman; N N Landman
Journal:  J Chem Educ       Date:  1976-09       Impact factor: 2.979

3.  A simplified method for cyanogen bromide activation of agarose for affinity chromatography.

Authors:  S C March; I Parikh; P Cuatrecasas
Journal:  Anal Biochem       Date:  1974-07       Impact factor: 3.365

4.  Stimulation of propionyl CoA and beta-methylcrotonyl CoA carboxylase activities in human leukocytes and cultured fibroblasts by biotin.

Authors:  B Wolf; L E Rosenberg
Journal:  Pediatr Res       Date:  1979-11       Impact factor: 3.756

5.  Isolation and characterization of propionyl-CoA carboxylase from normal human liver. Evidence for a protomeric tetramer of nonidentical subunits.

Authors:  F Kalousek; M D Darigo; L E Rosenberg
Journal:  J Biol Chem       Date:  1980-01-10       Impact factor: 5.157

Review 6.  The biotin-dependent enzymes.

Authors:  J Moss; M D Lane
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1971

7.  Biochemical differences between mutant propionyl-CoA carboxylases from two complementation groups.

Authors:  B Wolf; Y E Hsia; L E Rosenberg
Journal:  Am J Hum Genet       Date:  1978-09       Impact factor: 11.025

8.  Biotin-response organicaciduria. Multiple carboxylase defects and complementation studies with propionicacidemia in cultured fibroblasts.

Authors:  M Saunders; L Sweetman; B Robinson; K Roth; R Cohn; R A Gravel
Journal:  J Clin Invest       Date:  1979-12       Impact factor: 14.808

9.  Deficient acetyl CoA carboxylase activity in multiple carboxylase deficiency.

Authors:  G L Feldman; B Wolf
Journal:  Clin Chim Acta       Date:  1981-04-09       Impact factor: 3.786

10.  Use of avidin-sepharose to isolate and idenify biotin polypeptides from crude extracts.

Authors:  J A Swack; G L Zander; M F Utter
Journal:  Anal Biochem       Date:  1978-06-15       Impact factor: 3.365
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