Literature DB >> 6120174

A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells.

J J Lin, S A Queally.   

Abstract

We have obtained a hybridoma clone, JLJ5a, which secretes monospecific antibody directed against a 110-kdalton protein of gerbil fibroma cells, Rat-1 fibroblasts, and L6 myoblasts. It appears to be localized in the Golgi apparatus by the following criteria: (a) In double-staining experiments the localization of the 110-kdalton protein by the JLJ5a monoclonal antibody was coincident with the reaction products of thiamine pyrophosphatase (one of the enzyme markers of the Golgi apparatus; Novikoff and Goldfischer, 1961, Proc. Natl. Acad. Sci. U.S.A. 47:802-810) in the same cells. (b) The staining pattern of the JLJ5a monoclonal antibody became fragmented and dispersed into vacuoles after pretreatment of the cells with Colcemid or monensin.

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Year:  1982        PMID： 6120174      PMCID： PMC2112016          DOI： 10.1083/jcb.92.1.108

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  20 in total

Review 1.  Intracellular aspects of the process of protein synthesis.

Authors:  G Palade
Journal:  Science       Date:  1975-08-01       Impact factor: 47.728

2.  Evidence against interconversion of microtubules and filaments.

Authors:  M DeBrabander; F Aerts; R Van de Veire; M Borgers
Journal:  Nature       Date:  1975-01-10       Impact factor: 49.962

3.  Influence of colchicine and vinblastine on the golgi complex and matrix deposition in chondrocyte aggregates. An ultrastructural study.

Authors:  S Moskalewski; J Thyberg; S Lohmander; U Friberg
Journal:  Exp Cell Res       Date:  1975-10-15       Impact factor: 3.905

4.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

Review 5.  The Golgi apparatus: structure and function.

Authors:  H W Beams; R G Kessel
Journal:  Int Rev Cytol       Date:  1968

6.  In vitro influence of colchicine on the Golgi complex in A- and B-cells of guinea pig pancreatic islets.

Authors:  S Moskalewski; J Thyberg; U Friberg
Journal:  J Ultrastruct Res       Date:  1976-02

7.  Chemical mutagenesis at the phosphoribosyltransferase locus in cultured human lymphoblasts.

Authors:  K Sato; R S Slesinski; J W Littlefield
Journal:  Proc Natl Acad Sci U S A       Date:  1972-05       Impact factor: 11.205

8.  The ultrastructure of synchronized HeLa cells.

Authors:  R A Erlandson; E de Harven
Journal:  J Cell Sci       Date:  1971-03       Impact factor: 5.285

9.  Golgi apparatus, GERL, and lysosomes of neurons in rat dorsal root ganglia, studied by thick section and thin section cytochemistry.

Authors:  P M Novikoff; A B Novikoff; N Quintana; J J Hauw
Journal:  J Cell Biol       Date:  1971-09       Impact factor: 10.539

10.  Plasma cell immunoglobulin secretion: arrest is accompanied by alterations of the golgi complex.

Authors:  A M Tartakoff; P Vassalli
Journal:  J Exp Med       Date:  1977-11-01       Impact factor: 14.307
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  18 in total

1.  Helix pomatia agglutinin binds specifically to the Golgi apparatus in cultured human fibroblasts and reveals two Golgi apparatus-specific glycoproteins.

Authors:  I Virtanen
Journal:  Histochemistry       Date:  1990

2.  Role of microtubules in the distribution of the Golgi apparatus: effect of taxol and microinjected anti-alpha-tubulin antibodies.

Authors:  J Wehland; M Henkart; R Klausner; I V Sandoval
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

Review 3.  Endocytosis and the recycling of plasma membrane.

Authors:  R M Steinman; I S Mellman; W A Muller; Z A Cohn
Journal:  J Cell Biol       Date:  1983-01       Impact factor: 10.539

4.  Demonstration of a common antigenic site on endomembrane proteins of Phaseolus vulgaris by a rat monoclonal antibody : Tentative identification of arabinan synthase and consequences for its regulation.

Authors:  G P Bolwell; D H Northcote
Journal:  Planta       Date:  1984-09       Impact factor: 4.116

5.  Effects of small doses of cytochalasins on fibroblasts: preferential changes of active edges and focal contacts.

Authors:  L V Domnina; V I Gelfand; O Y Ivanova; E V Leonova; O Y Pletjushkina; J M Vasiliev; I M Gelfand
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

6.  Anti-golgi complex autoantibodies in a patient with Sjögren syndrome and lymphoma.

Authors:  J L Rodríguez; C Gelpi; T M Thomson; F J Real; J Fernández
Journal:  Clin Exp Immunol       Date:  1982-09       Impact factor: 4.330

7.  A monoclonal antibody against a 135-K Golgi membrane protein.

Authors:  B Burke; G Griffiths; H Reggio; D Louvard; G Warren
Journal:  EMBO J       Date:  1982       Impact factor: 11.598

8.  Associations of elements of the Golgi apparatus with microtubules.

Authors:  A A Rogalski; S J Singer
Journal:  J Cell Biol       Date:  1984-09       Impact factor: 10.539

9.  Two integral membrane proteins located in the cis-middle and trans-part of the Golgi system acquire sialylated N-linked carbohydrates and display different turnovers and sensitivity to cAMP-dependent phosphorylation.

Authors:  L Yuan; J G Barriocanal; J S Bonifacino; I V Sandoval
Journal:  J Cell Biol       Date:  1987-07       Impact factor: 10.539

10.  Endogenous and monoclonal antibodies to the rat pancreatic acinar cell Golgi complex.

Authors:  Z D Smith; F D'Eugenio-Gumkowski; K Yanagisawa; J D Jamieson
Journal:  J Cell Biol       Date:  1984-06       Impact factor: 10.539
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