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Literature DB >> 6111746

Physical and functional mapping of rat-liver mitochondrial DNA.

T B Kazakova, S G Babich, M P Mel'nikova, N V Tzimbalenko, E A Braga, V V Nosikov.

Abstract

Rat-liver mitochondrial DNA (mtDNA) contains 2 cleavage sites of the restriction endonuclease Xbal. The molecular sizes of restriction fragments are 6.6 X 10(6) and 3.7 X 10(6) D. The results of partial cleavage of mtDNA with EcoRI allow the fragment F (0.32 X 10(6) D) to be localized in the sequence ABCEGFHDA. The functional map of mtDNA is constructed for two genes of the ATP-ase mRNAs from rat-liver mitochondria. Molecular hybridization shows that the ATPase genes are located in fragment B and in the GEHD area of mtDNA EcoRI cleavage.

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Year: 1981 PMID： 6111746 DOI： 10.1007/bf02358186

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

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References

20 in total

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Physical and functional mapping of rat-liver mitochondrial DNA.

1. RNA - DNA hybridization on membrane filters with fragmented mammalian DNA.

2. Assembly of the mitochondrial membrane system: partial sequence of a mitochondrial ATPase gene in Saccharomyces cerevisiae.

3. The dicyclohexylcarbodiimide-binding protein of rat liver mitochondria as a product of the mitochondrial protein synthesis.

4. Diffusion-in-gel methods for immunological analysis. II.

5. Transcription in yeast mitochondria: isolation and physical mapping of messenger RNAs for subunits of cytochrome c oxidase and ATPase.

6. EcoRI cleavage site variants of mitochondrial DNA molecules from rats.

7. The genetic localization of presumptive mitochondrial messenger RNAs on rat-liver mitochondrial DNA.

8. Hybridization of RNA labelled with 125 I to high specific activity.

9. [Localization of the recognition sites of BamHI, HpaI and EcoRI restrictases on rat liver mitochondrial DNA].

10. Restriction endonuclease cleavage maps of rat and mouse mitochondrial DNAs.