Literature DB >> 6110205

The construction of a recombinant cDNA library representative of the poly(A)+ mRNA population from normal human lymphocytes.

D Woods, J Crampton, B Clarke, R Williamson.   

Abstract

A recombinant library has been constructed using the plasmid pAT153 and double stranded cDNA prepared from normal human lymphocyte poly(A)+ RNA. Transformation conditions were optimized to yield approximately 200,000 recombinants per microgram of double stranded cDNA. Statistical analysis as well as sequence complexity analysis of the inserted sequences indicates that the cDNA library is representative of > 99% of the poly(A)+ RNA present in the normal human lymphocyte.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 6110205      PMCID： PMC324291          DOI： 10.1093/nar/8.22.5157

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  23 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Reversal of bacteriophage T4 induced polynucleotide kinase action.

Authors:  J H van de Sande; K Kleppe; H G Khorana
Journal:  Biochemistry       Date:  1973-12-04       Impact factor: 3.162

3.  Polyadenylic acid-containing RNA in Xenopus laevis oocytes.

Authors:  M Rosbash
Journal:  J Mol Biol       Date:  1974-05-05       Impact factor: 5.469

4.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

5.  Tissue typing using a routine one-step lymphocyte separation procedure.

Authors:  R Harris; E O Ukaejiofo
Journal:  Br J Haematol       Date:  1970-02       Impact factor: 6.998

6.  A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.

Authors:  R Radloff; W Bauer; J Vinograd
Journal:  Proc Natl Acad Sci U S A       Date:  1967-05       Impact factor: 11.205

7.  A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.

Authors:  P C Wensink; D J Finnegan; J E Donelson; D S Hogness
Journal:  Cell       Date:  1974-12       Impact factor: 41.582

8.  Proteolytic cleavage fo native DNA polymerase into two different catalytic fragments. Influence of assay condtions on the change of exonuclease activity and polymerase activity accompanying cleavage.

Authors:  H Klenow; K Overgaard-Hansen; S A Patkar
Journal:  Eur J Biochem       Date:  1971-10-14

9.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

10.  Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.

Authors:  R Roychoudhury; E Jay; R Wu
Journal:  Nucleic Acids Res       Date:  1976-01       Impact factor: 16.971
View more
  16 in total

1.  The occurrence of families of repetitive sequences in a library of cloned cDNA from human lymphocytes.

Authors:  J M Crampton; K E Davies; T F Knapp
Journal:  Nucleic Acids Res       Date:  1981-08-11       Impact factor: 16.971

2.  Isolation and characterization of a cDNA clone for bovine cytochrome c oxidase subunit IV.

Authors:  M I Lomax; N J Bachman; M S Nasoff; M H Caruthers; L I Grossman
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

3.  The effects of lectin transformation on cytoplasmic polyadenylated RNA from human lymphocytes.

Authors:  E McCairns; D Fahey; G E Muscat; P B Rowe
Journal:  Mol Cell Biochem       Date:  1983       Impact factor: 3.396

4.  Sequence complexity and diversity of polyadenylated RNA molecules of human normal resting, PHA-stimulated and leukemic lymphocytes.

Authors:  G Torelli; S Ferrari; A Donelli; R Cadossi; S Ferrari; P Bosi; U Torelli
Journal:  Nucleic Acids Res       Date:  1981-12-21       Impact factor: 16.971

5.  Inter-locus and intra-allelic polymorphisms of HLA class I antigen gene mRNA.

Authors:  D Arnot; J W Lillie; C Auffray; D Kappes; J L Strominger
Journal:  Immunogenetics       Date:  1984       Impact factor: 2.846

6.  There are approximately 20 actin gene in the human genome.

Authors:  S E Humphries; R Whittall; A Minty; M Buckingham; R Williamson
Journal:  Nucleic Acids Res       Date:  1981-10-10       Impact factor: 16.971

7.  The complete nucleotide sequence of the RNA coding for the primary translation product of foot and mouth disease virus.

Authors:  A R Carroll; D J Rowlands; B E Clarke
Journal:  Nucleic Acids Res       Date:  1984-03-12       Impact factor: 16.971

8.  Identification and isolation of transcribed human X chromosome DNA sequences.

Authors:  L M Kunkel; U Tantravahi; D M Kurnit; M Eisenhard; G P Bruns; S A Latt
Journal:  Nucleic Acids Res       Date:  1983-11-25       Impact factor: 16.971

9.  Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product.

Authors:  D E Woods; A F Markham; A T Ricker; G Goldberger; H R Colten
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

10.  Benzpyrene groups bind preferentially to the DNA of active chromatin in human lung cells.

Authors:  J E Arrand; A M Murray
Journal:  Nucleic Acids Res       Date:  1982-03-11       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.