Literature DB >> 6109516

Non-immunological precipitation by the neutral detergent triton X-100 in agar gel diffusion.

B J Mansheim, M L Stenstrom.   

Abstract

Triton X-100 can be used to clarify vague immunoprecipitin lines from bacterial antigens; however, non-immunological precipitation can lead to mistaken interpretation of immunodiffusion results. If Triton X-100 is added directly to the gel during preparation rather than to the antigen well, this detergent artifact can be eliminated.

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Year:  1980        PMID： 6109516      PMCID： PMC291734          DOI： 10.1128/aem.40.6.1139-1141.1980

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  6 in total

1.  Purification and immunochemical characterization of the outer membrane complex of Bacteroides melaninogenicus subspecies asaccharolyticus.

Authors:  B J Mansheim; D L Kasper
Journal:  J Infect Dis       Date:  1977-05       Impact factor: 5.226

2.  Detergent-containing gels for immunological studies of solubilized erythrocyte membrane components.

Authors:  O J Bjerrum; P Lundahl
Journal:  Scand J Immunol Suppl       Date:  1973

3.  Non-immunological precipitation of serum by sodium dodecyl sulfate in agar diffusion.

Authors:  C T Cho; K K Feng
Journal:  Appl Microbiol       Date:  1974-10

4.  Nonspecific precipitation of serum proteins by sodium lauryl sulfate in agar diffusion and immunoelectrophoresis.

Authors:  E L Palmer; M L Martin; J C Hierholzer; D W Ziegler
Journal:  Appl Microbiol       Date:  1971-05

5.  Identification of a subspecies-specific capsular antigen from Bacteroides melaninogenicus subspecies asaccharolyticus by immunofluorescence and electron microscopy.

Authors:  B J Mansheim; C A Solstad; D L Kasper
Journal:  J Infect Dis       Date:  1978-12       Impact factor: 5.226

6.  Detection of anticapsular antibodies to Bacteroides asaccharolyticus in serum from rabbits and humans by use of an enzyme-linked immunosorbent assay.

Authors:  B J Mansheim; D L Kasper
Journal:  J Infect Dis       Date:  1979-12       Impact factor: 5.226
  6 in total

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