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Literature DB >> 6108994

Isolation and characterization of brain endothelial cells: morphology and enzyme activity.

S K Williams, J F Gillis, M A Matthews, R C Wagner, M W Bitensky.

Abstract

Microvessels were isolated from rat brain using a double collagenase treatment which removed the endothelial basement membranes. The isolate was characterized by intact luminal and abluminal membranes and an absence of pericytes and astrocyte membranes. Minimal contamination by 5'-nucleotidase, an enzyme believed exclusively localized within the plasma membranes of neuroglia, established the purity of the isolated microvessels. Enrichment of alkaline phosphatase and gamma-glutamyl transpeptidase activity in microvessel preparations supports the endothelial localization of these enzymes.

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Year: 1980 PMID： 6108994 DOI： 10.1111/j.1471-4159.1980.tb06274.x

Source DB: PubMed Journal: J Neurochem ISSN： 0022-3042 Impact factor: 5.372

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Cited

33 in total

Review 1. Drug transport across the blood-brain barrier. II. Experimental techniques to study drug transport.

Authors: J B Van Bree; A G De Boer; M Danhof; D D Breimer
Journal: Pharm Weekbl Sci Date: 1992-12-11

2. Biomedical Technologies for in vitro Screening and Controlled Delivery of Neuroactive Compounds.

Authors: John P Frampton; Michael L Shuler; William Shain; Matthew R Hynd
Journal: Cent Nerv Syst Agents Med Chem Date: 2008

Review 3. The use of cultured epithelial and endothelial cells for drug transport and metabolism studies.

Authors: K L Audus; R L Bartel; I J Hidalgo; R T Borchardt
Journal: Pharm Res Date: 1990-05 Impact factor: 4.200

4. Characterization of gamma-glutamyl transpeptidase activity of cultured endothelial cells from porcine brain capillaries.

Authors: U Mischeck; J Meyer; H J Galla
Journal: Cell Tissue Res Date: 1989-04 Impact factor: 5.249

Isolation and characterization of brain endothelial cells: morphology and enzyme activity.

Review 1. Drug transport across the blood-brain barrier. II. Experimental techniques to study drug transport.

2. Biomedical Technologies for in vitro Screening and Controlled Delivery of Neuroactive Compounds.

Review 3. The use of cultured epithelial and endothelial cells for drug transport and metabolism studies.

4. Characterization of gamma-glutamyl transpeptidase activity of cultured endothelial cells from porcine brain capillaries.

5. Specific antigen and organelle expression of a long-term rhesus endothelial cell line.

6. The formation of fenestrations and channels by capillary endothelium in vitro.

7. Phenotypic diversity in cultured cerebral microvascular endothelial cells.

8. Long-term serial cultivation of arterial and capillary endothelium from adult bovine brain.

9. Pharmacological properties of voltage-dependent calcium channels in functional microvessels isolated from rat brain.

10. Ultrastructural cytochemical studies of cerebral microvasculature in scrapie infected mice.