| Literature DB >> 610874 |
Abstract
Both the number of colonies formed from single cells and their average size are affected by the temperature at which nontransformed human and chicken fibroblasts are harvested from monolayer cultures. At constant levels of trypsin activity, treatment of cells at temperatures less than 15 degrees C during all steps from monolayer to subsequent culture markedly improves viability and multiplication potential of single cells.Entities:
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Year: 1977 PMID： 610874 DOI： 10.1016/0309-1651(77)90063-7
Source DB: PubMed Journal: Cell Biol Int Rep ISSN： 0309-1651