Literature DB >> 6105618

Identification of a tetrodotoxin-sensitive Na+ channel in a variety in fibroblast lines.

J Pouysségur, Y Jacques, M Lazdunski.   

Abstract

The action potential Na+ ionophore of excitable cells can be activated either by alkaloid compounds such as veratridine, or by small polypeptide toxins extracted from scorpion venom or sea anemone. One of the main features of this Na+ channel is that it is blocked by tetrodotoxin (TTX). However, we report here that during analysis of Na+ influx in resting fibroblasts, we found that a variety of fibroblast lines also possess a TTX receptor. Veratridine and sea anemone toxin act synergistically to stimulate Na+ influx 7 to 10-fold in hamster and rat fibroblasts. As in excitable cells, this toxin-stimulated Na+ influx is blocked by TTX. Addition of serum to hamster fibroblasts arrested in G0 stimulates Na+ influx three-fold. Observations that TTX does not prevent serum-activated Na+ influx, initiation of DNA synthesis and cell proliferation suggest that the fast Na+ channel which we have identified in fibroblasts is not involved in growth control.

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Year:  1980        PMID： 6105618     DOI： 10.1038/286162a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  10 in total

1.  The sodium channels of the neuroblastoma x glioma 108 CC 15 hybrid cell change their sensitivity for volatile and local anesthetics upon continuous passage.

Authors:  P W Tas; H G Kress; K Koschel
Journal:  J Neural Transm       Date:  1989       Impact factor: 3.575

2.  Voltage-dependent sodium and potassium channels in mammalian cultured Schwann cells.

Authors:  P Shrager; S Y Chiu; J M Ritchie
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

3.  A specific mutation abolishing Na+/H+ antiport activity in hamster fibroblasts precludes growth at neutral and acidic pH.

Authors:  J Pouysségur; C Sardet; A Franchi; G L'Allemain; S Paris
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205

4.  Na+-channel-associated scorpion toxin receptor sites as probes for neuronal evolution in vivo and in vitro.

Authors:  Y Berwald-Netter; N Martin-Moutot; A Koulakoff; F Couraud
Journal:  Proc Natl Acad Sci U S A       Date:  1981-02       Impact factor: 11.205

5.  Growth factor activation of an amiloride-sensitive Na+/H+ exchange system in quiescent fibroblasts: coupling to ribosomal protein S6 phosphorylation.

Authors:  J Pouysségur; J C Chambard; A Franchi; S Paris; E Van Obberghen-Schilling
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

6.  Cell growth and net Na+ flux are inhibited by a protein produced by kidney epithelial cells in culture.

Authors:  M M Walsh-Reitz; F G Toback; R W Holley
Journal:  Proc Natl Acad Sci U S A       Date:  1984-02       Impact factor: 11.205

7.  Extraneuronal saxitoxin binding sites in rabbit myelinated nerve.

Authors:  J M Ritchie; H P Rang
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205

8.  Differentiation of the fast Na+ channel in embryonic heart cells: interaction of the channel with neurotoxins.

Authors:  J F Renaud; G Romey; A Lombet; M Lazdunski
Journal:  Proc Natl Acad Sci U S A       Date:  1981-09       Impact factor: 11.205

9.  Different functional states of tetrodotoxin sensitive and tetrodotoxin resistant Na+ channels occur during the in vitro development of rat skeletal muscle.

Authors:  C Frelin; H P Vijverberg; G Romey; P Vigne; M Lazdunski
Journal:  Pflugers Arch       Date:  1984-10       Impact factor: 3.657

10.  Sodium-channels in non-excitable glioma cells, shown by the influence of veratridine, scorpion toxin, and tetrodotoxin on membrane potential and on ion transport.

Authors:  G Reiser; B Hamprecht
Journal:  Pflugers Arch       Date:  1983-06-01       Impact factor: 3.657
  10 in total

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