| Literature DB >> 6104515 |
D Fleischman, M Denisevich, D Raveed, R G Pannbacker.
Abstract
Axonemes were isolated from purified bovine retinal rod outer segments by dissolving the outer segment membranes in detergent and separating the axonemes by centrifugation on a linear detergent-containing sucrose density gradient. Guanylate cyclase (GTP pyrophosphate-lyase (cyclizing), EC 4.61.2) activity was concentrated in the axoneme fraction. Guanylate cyclase eluted in the void volume when detergent-solubilized rod outer segments were subjected to exclusion chromatography on Sepharose 4B. Attempts to extract guanylate cyclase from isolated axonemes with salt, EDTA, base and other reagents were successful.Entities:
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Year: 1980 PMID: 6104515 DOI: 10.1016/0304-4165(80)90419-5
Source DB: PubMed Journal: Biochim Biophys Acta ISSN: 0006-3002