Literature DB >> 6102984

Purification of glutamine synthetase from a variety of bacteria.

S L Streicher, B Tyler.   

Abstract

We have developed two procedures which allow the very rapid purification of glutamine synthetase (GS) from a diverse variety of bacteria. The first procedure, based upon differential sedimentation, depends upon the association of GS with deoxyribonucleic acid in cell extracts. The second procedure, derived from the method of C. Gross et al (J. Bacteriol. 128:382-389, 1976) for purifying ribonucleic acid polymerase by polyethylene glycol (PEG) precipitation, enabled us to obtain high yields of GS from either small or large quantities of cells. We used the PEG procedure to purify GS from Klebsiella aerogenes, K. pneumoniae, Escherichia coli, Salmonella typhimurium, Rhizobium sp. strain 32H1, R. meliloti, Azotobacter vinelandii, Pseudomonas putida, Caulobacter crescentus, and Rhodopseudomonas capsulata. The purity of the GS obtained, judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was high, and in many instances only a single protein band was detected.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 6102984      PMCID： PMC293904          DOI： 10.1128/jb.142.1.69-78.1980

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  34 in total

1.  Identification of the structural gene for glutamine synthetase in Klebsiella aerogenes.

Authors:  A B Deleo; B Magasanik
Journal:  J Bacteriol       Date:  1975-01       Impact factor: 3.490

2.  Glutamate synthase from Escherichia coli. An iron-sulfide flavoprotein.

Authors:  R E Miller; E R Stadtman
Journal:  J Biol Chem       Date:  1972-11-25       Impact factor: 5.157

3.  Non-specific DNA binding of genome regulating proteins as a biological control mechanism: I. The lac operon: equilibrium aspects.

Authors:  P H von Hippel; A Revzin; C A Gross; A C Wang
Journal:  Proc Natl Acad Sci U S A       Date:  1974-12       Impact factor: 11.205

4.  Purification and DNA-binding properties of the catabolite gene activator protein.

Authors:  A D Riggs; G Reiness; G Zubay
Journal:  Proc Natl Acad Sci U S A       Date:  1971-06       Impact factor: 11.205

5.  The binding of cyclic adenosine monophosphate receptor to deoxyribonucleic acid.

Authors:  P Nissley; W B Anderson; M Gallo; I Pastan; R L Perlman
Journal:  J Biol Chem       Date:  1972-07-10       Impact factor: 5.157

6.  Regulation of glutamine synthetase. I. Purification and properties of glutamine synthetase from Escherichia coli.

Authors:  C A Woolfolk; B Shapiro; E R Stadtman
Journal:  Arch Biochem Biophys       Date:  1966-09-26       Impact factor: 4.013

7.  Activation of transcription of hut DNA by glutamine synthetase.

Authors:  B Tyler; A B Deleo; B Magasanik
Journal:  Proc Natl Acad Sci U S A       Date:  1974-01       Impact factor: 11.205

8.  Regulation of the synthesis of enzymes responsible for glutamate formation in Klebsiella aerogenes.

Authors:  J E Brenchley; M J Prival; B Magasanik
Journal:  J Biol Chem       Date:  1973-09-10       Impact factor: 5.157

9.  Zinc-induced paracrystalline aggregation of glutamine synthetase.

Authors:  R E Miller; E Shelton; E R Stadtman
Journal:  Arch Biochem Biophys       Date:  1974-07       Impact factor: 4.013

10.  Genetic control of glutamine synthetase in Klebiella aerogenes.

Authors:  S L Streicher; R A Bender; B Magasanik
Journal:  J Bacteriol       Date:  1975-01       Impact factor: 3.490
View more
  13 in total

1.  Cloning and nucleotide sequence of an archaebacterial glutamine synthetase gene: phylogenetic implications.

Authors:  A M Sanangelantoni; D Barbarini; G Di Pasquale; P Cammarano; O Tiboni
Journal:  Mol Gen Genet       Date:  1990-04

2.  Cloning, Expression, and Purification of Glutamine Synthetase from Clostridium acetobutylicum.

Authors:  K P Usdin; H Zappe; D T Jones; D R Woods
Journal:  Appl Environ Microbiol       Date:  1986-09       Impact factor: 4.792

3.  Purification and characterisation of glutamine synthetase from Nocardia corallina.

Authors:  N Illing; R T Hill; D R Woods
Journal:  Antonie Van Leeuwenhoek       Date:  1988       Impact factor: 2.271

4.  Characterization of recombinant glutamine synthetase from the hyperthermophilic archaeon Pyrococcus sp. strain KOD1.

Authors:  R N Adul Rahman; B Jongsareejit; S Fujiwara; T Imanaka
Journal:  Appl Environ Microbiol       Date:  1997-06       Impact factor: 4.792

5.  Purification and characterization of glutamine synthetase from the archaebacterium Methanobacterium ivanovi.

Authors:  L Bhatnagar; J G Zeikus; J P Aubert
Journal:  J Bacteriol       Date:  1986-02       Impact factor: 3.490

6.  Polarity in the glnA operon: suppression of the reg- phenotype by rho mutations.

Authors:  S K Guterman; G Roberts; B Tyler
Journal:  J Bacteriol       Date:  1982-06       Impact factor: 3.490

7.  A nonsense mutation in the structural gene for glutamine synthetase leading to loss of nitrogen regulation in Klebsiella aerogenes.

Authors:  R A Bender; L J Eades
Journal:  Mol Gen Genet       Date:  1982

8.  Glutamine synthetase of pseudomonads: some biochemical and physicochemical properties.

Authors:  J M Meyer; E R Stadtman
Journal:  J Bacteriol       Date:  1981-05       Impact factor: 3.490

9.  Properties of the Bacillus licheniformis A5 glutamine synthetase purified from cells grown in the presence of ammonia or nitrate.

Authors:  T J Donohue; R W Bernlohr
Journal:  J Bacteriol       Date:  1981-08       Impact factor: 3.490

10.  Is hydrogen peroxide involved in the benzyl viologen-mediated in-vivo inactivation of rat liver glutamine synthetase?

Authors:  F J Muriana; V Ruiz-Gutierrez; A M Relimpio
Journal:  Int J Exp Pathol       Date:  1993-04       Impact factor: 1.925
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.