Literature DB >> 6099060

Continuous spectrophotometric assay for aminoacyl-tRNA synthetases.

G G Chang, F Pan, Y H Lin, H Y Wang.   

Abstract

A simple, continuous assay for aminoacyl-tRNA synthetases utilizing a commercially available pyrophosphate assay reagent kit was demonstrated. The method coupled aminoacyl-tRNA synthetase activity with pyrophosphate-dependent fructose-6-phosphate kinase, aldolase, triosephosphate isomerase, and glycerophosphate dehydrogenase. PPi formation was correlated with the oxidation of NADH, and was monitored continuously by the decrease of absorbance at 340 nm.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6099060     DOI： 10.1016/0003-2697(84)90478-0

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  2 in total

1.  Pontocerebellar hypoplasia type 6 caused by mutations in RARS2: definition of the clinical spectrum and molecular findings in five patients.

Authors:  Denise Cassandrini; Maria Roberta Cilio; Marzia Bianchi; Mara Doimo; Martina Balestri; Alessandra Tessa; Teresa Rizza; Geppo Sartori; Maria Chiara Meschini; Claudia Nesti; Giulia Tozzi; Vittoria Petruzzella; Fiorella Piemonte; Luigi Bisceglia; Claudio Bruno; Carlo Dionisi-Vici; Adele D'Amico; Fabiana Fattori; Rosalba Carrozzo; Leonardo Salviati; Filippo M Santorelli; Enrico Bertini
Journal:  J Inherit Metab Dis       Date:  2012-05-08       Impact factor: 4.982

2.  Zooplankton Growth, Respiration and Grazing on the Australian Margins of the Tropical Indian and Pacific Oceans.

Authors:  A David McKinnon; Jason Doyle; Samantha Duggan; Murray Logan; Christian Lønborg; Richard Brinkman
Journal:  PLoS One       Date:  2015-10-15       Impact factor: 3.240
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.