Literature DB >> 6096220

High-copy-number derivatives of the plasmid cloning vector pBR322.

I Boros, G Pósfai, P Venetianer.   

Abstract

A stable copy-number mutant of a pBR322-derived plasmid was isolated. The mutation was found to be a single G----T transversion located near the 3' end of a DNA segment coding for the regulatory RNA I. The resulting copy number for this plasmid is approx. 1000 per cell or 65% of total cellular DNA. Several cloning vectors have been constructed from this copy-number mutant and their practical application is discussed.

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Year:  1984        PMID： 6096220     DOI： 10.1016/0378-1119(84)90130-6

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  29 in total

1.  A cis-acting mutation in the Sindbis virus junction region which affects subgenomic RNA synthesis.

Authors:  A Grakoui; R Levis; R Raju; H V Huang; C M Rice
Journal:  J Virol       Date:  1989-12       Impact factor: 5.103

2.  Nucleotide sequence and genome organization of canine parvovirus.

Authors:  A P Reed; E V Jones; T J Miller
Journal:  J Virol       Date:  1988-01       Impact factor: 5.103

3.  Construction of a Co1E1 plasmid bearing inducible high-copy-number phenotype.

Authors:  D Bachvarov; E Jay; I Ivanov
Journal:  Folia Microbiol (Praha)       Date:  1990       Impact factor: 2.099

4.  Rapid and simple method for purification of nucleic acids.

Authors:  R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

5.  Adsorption of plasmid DNA to mineral surfaces and protection against DNase I.

Authors:  G Romanowski; M G Lorenz; W Wackernagel
Journal:  Appl Environ Microbiol       Date:  1991-04       Impact factor: 4.792

6.  Microisolation of yeast nucleic acids on the microtitre plate without using lytic enzymes.

Authors:  M Pospísek; Z Palková
Journal:  Nucleic Acids Res       Date:  1991-09-25       Impact factor: 16.971

7.  Tn1000 insertional mutagenesis of cloned repressor gene of the phage L: plasmid oligomerization in the presence of F'lac.

Authors:  P Karlovský; M Vasková
Journal:  Folia Microbiol (Praha)       Date:  1987       Impact factor: 2.099

8.  RecBCD, SbcCD and ExoI process a substrate created by convergent replisomes to complete DNA replication.

Authors:  Nicklas A Hamilton; Brian M Wendel; Emma A Weber; Charmain T Courcelle; Justin Courcelle
Journal:  Mol Microbiol       Date:  2019-05-06       Impact factor: 3.501

9.  Complex interaction of yeast nuclear proteins with the enhancer/promoter region of SV40.

Authors:  J Gyuris; L Dencsö; K Polyák; E Duda
Journal:  Curr Genet       Date:  1991-11       Impact factor: 3.886

10.  Identification and initial utilization of a portion of the smaller plasmid of Anabaena variabilis ATCC 29413 capable of replication in Anabaena sp. strain M-131.

Authors:  M A Murry; C P Wolk
Journal:  Mol Gen Genet       Date:  1991-05
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