Literature DB >> 6096099

Chromogenic immunodetection of human serum albumin and alpha-L-fucosidase clones in a human hepatoma cDNA expression library.

J R de Wet, H Fukushima, N N Dewji, E Wilcox, J S O'Brien, D R Helinski.   

Abstract

A human hepatoma cDNA library was constructed in lambda gt11, a bacteriophage vector that was designed to express cDNA-encoded antigenic determinants in Escherichia coli. The cDNA expression library contained approximately 8 X 10(6) recombinant phages with an average insert size of 780 bp. The feasibility of using a chromogenic immunodetection procedure to isolate cDNA clones was proved by isolating a human serum albumin (HSA) cDNA clone. An approximately 1.0-kb cDNA clone was then isolated by screening the library with rabbit anti-human alpha-L-fucosidase antibodies. The identity of the alpha-L-fucosidase cDNA clone was confirmed by DNA sequence analysis and a comparison of the predicted amino acid sequence to the amino acid sequences of human alpha-L-fucosidase tryptic peptides.

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Year:  1984        PMID： 6096099     DOI： 10.1089/dna.1.1984.3.437

Source DB:  PubMed          Journal:  DNA        ISSN： 0198-0238


  34 in total

1.  Processing of Alzheimer's disease-associated beta-amyloid precursor protein.

Authors:  N N Dewji; E R Shelton; M J Adler; H W Chan; J E Seegmiller; C Coronel
Journal:  J Mol Neurosci       Date:  1990       Impact factor: 3.444

2.  Nucleotide sequence of cloned cDNA for human sphingolipid activator protein 1 precursor.

Authors:  N N Dewji; D A Wenger; J S O'Brien
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

3.  Identification of a mutation in the structural alpha-L-fucosidase gene in fucosidosis.

Authors:  P J Willems; J K Darby; R A DiCioccio; P Nakashima; C Eng; K A Kretz; L L Cavalli-Sforza; E M Shooter; J S O'Brien
Journal:  Am J Hum Genet       Date:  1988-11       Impact factor: 11.025

4.  Cinnamyl-alcohol dehydrogenase, a molecular marker specific for lignin synthesis: cDNA cloning and mRNA induction by fungal elicitor.

Authors:  M H Walter; J Grima-Pettenati; C Grand; A M Boudet; C J Lamb
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205

5.  Cloning of cDNA to rat mammary-gland fatty acid synthase mRNA. Evidence for the expression of two mRNA species during lactation.

Authors:  M Braddock; D G Hardie
Journal:  Biochem J       Date:  1988-01-15       Impact factor: 3.857

6.  Molecular cloning of a cDNA for human alpha-L-fucosidase.

Authors:  H Fukushima; J R de Wet; J S O'Brien
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

7.  Isolation of human cDNA clones of myb-related genes, A-myb and B-myb.

Authors:  N Nomura; M Takahashi; M Matsui; S Ishii; T Date; S Sasamoto; R Ishizaki
Journal:  Nucleic Acids Res       Date:  1988-12-09       Impact factor: 16.971

8.  Evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.

Authors:  M A Gitt; S H Barondes
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

9.  Cloning of a cDNA encoding the human cation-dependent mannose 6-phosphate-specific receptor.

Authors:  R Pohlmann; G Nagel; B Schmidt; M Stein; G Lorkowski; C Krentler; J Cully; H E Meyer; K H Grzeschik; G Mersmann
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

10.  cDNA and protein sequence of polymorphic macaque albumins that differ in bilirubin binding.

Authors:  S Watkins; Y Sakamoto; J Madison; E Davis; D G Smith; J Dwulet; F W Putnam
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-15       Impact factor: 11.205
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