Literature DB >> 6095817

Azotobacter chroococcum 7Fe ferredoxin. Two pH-dependent forms of the reduced 3Fe clusters and its conversion to a 4Fe cluster.

S J George, A J Richards, A J Thomson, M G Yates.   

Abstract

Ferredoxin from Azotobacter chroococcum has been studied by low-temperature magnetic-circular-dichroism and electron-paramagnetic-resonance spectroscopy. When aerobically isolated ferredoxin contains a [3Fe-4S] and [4Fe-4S] cluster. Anaerobic treatment with dithionite in the presence of ethanediol reduces the [3Fe-4S] cluster to give two spectroscopically distinct forms RI and RII which are reversibly interconvertible with a pKa approximately 7.5. The higher-pH form, RII, has a high affinity for ferrous ion and converts readily to a [4Fe-4S]1+ cluster, scavenging iron from the medium. The presence of the iron chelator EDTA inhibits this conversion.

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Year:  1984        PMID： 6095817      PMCID： PMC1144420          DOI： 10.1042/bj2240247

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  19 in total

1.  On the nature of the spin coupling between the iron-sulfur clusters in the eight-iron ferredoxins.

Authors:  R Mathews; S Charlton; R H Sands; G Palmer
Journal:  J Biol Chem       Date:  1974-07-10       Impact factor: 5.157

2.  Non-heme iron (iron-sulfur) proteins of Azotobacter vinelandii.

Authors:  Y I Shethna
Journal:  Biochim Biophys Acta       Date:  1970-04-07

3.  The three-iron cluster in a ferredoxin from Desulphovibrio gigas. A low-temperature magnetic circular dichroism study.

Authors:  A J Thomson; A E Robinson; M K Johnson; J J Moura; I Moura; A V Xavier; J Legall
Journal:  Biochim Biophys Acta       Date:  1981-08-28

4.  Mössbauer studies of beef heart aconitase: evidence for facile interconversions of iron-sulfur clusters.

Authors:  T A Kent; J L Dreyer; M C Kennedy; B H Huynh; M H Emptage; H Beinert; E Münck
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

5.  Characterization of the Fe-S cluster in aconitase using low temperature magnetic circular dichroism spectroscopy.

Authors:  M K Johnson; A J Thomson; A J Richards; J Peterson; A E Robinson; R R Ramsay; T P Singer
Journal:  J Biol Chem       Date:  1984-02-25       Impact factor: 5.157

6.  On the nature of the iron-sulfur centers in a ferredoxin from Azotobacter vinelandii. Mössbauer studies and cluster displacement experiments.

Authors:  M H Emptage; T A Kent; B H Huynh; J Rawlings; W H Orme-Johnson; E Münck
Journal:  J Biol Chem       Date:  1980-03-10       Impact factor: 5.157

7.  High and low reduction potential 4Fe-4S clusters in Azotobacter vinelandii (4Fe-4S) 2ferredoxin I. Influence of the polypeptide on the reduction potentials.

Authors:  W V Sweeney; J C Rabinowitz; D C Yoch
Journal:  J Biol Chem       Date:  1975-10-10       Impact factor: 5.157

8.  The electron transport system in nitrogen fixation by Azotobacter. II. Isolation and function of a new type of ferredoxin.

Authors:  D C Yoch; J R Benemann; R C Valentine; D I Arnon
Journal:  Proc Natl Acad Sci U S A       Date:  1969-12       Impact factor: 11.205

9.  Spectroscopic studies of ferricyanide oxidation of Azotobacter vinelandii ferredoxin I.

Authors:  T V Morgan; P J Stephens; F Devlin; C D Stout; K A Melis; B K Burgess
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

10.  Iron-sulfur stoichiometry and structure of iron-sulfur clusters in three-iron proteins: evidence for [3Fe-4S] clusters.

Authors:  H Beinert; M H Emptage; J L Dreyer; R A Scott; J E Hahn; K O Hodgson; A J Thomson
Journal:  Proc Natl Acad Sci U S A       Date:  1983-01       Impact factor: 11.205
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  4 in total

1.  Magnetic circular dichroism study of the selenium-substituted form (Fe3Se4) of bovine heart aconitase.

Authors:  J L Breton; J A Farrar; M C Kennedy; H Beinert; A J Thomson
Journal:  Biochem J       Date:  1995-10-01       Impact factor: 3.857

2.  Electrochemical and spectroscopic characterization of the 7Fe form of ferredoxin III from Desulfovibrio africanus.

Authors:  F A Armstrong; S J George; R Cammack; E C Hatchikian; A J Thomson
Journal:  Biochem J       Date:  1989-11-15       Impact factor: 3.857

3.  Voltammetric studies of the reactions of iron-sulphur clusters ([3Fe-4S] or [M3Fe-4S]) formed in Pyrococcus furiosus ferredoxin.

Authors:  S E Fawcett; D Davis; J L Breton; A J Thomson; F A Armstrong
Journal:  Biochem J       Date:  1998-10-15       Impact factor: 3.857

4.  Second gene (nifH*) coding for a nitrogenase iron protein in Azotobacter chroococcum is adjacent to a gene coding for a ferredoxin-like protein.

Authors:  R Robson; P Woodley; R Jones
Journal:  EMBO J       Date:  1986-06       Impact factor: 11.598
  4 in total

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