| Literature DB >> 6095494 |
A R Dluzewski, I T Ling, K Rangachari, P A Bates, R J Wilson.
Abstract
Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.Entities:
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Year: 1984 PMID： 6095494 DOI： 10.1016/0035-9203(84)90221-9
Source DB: PubMed Journal: Trans R Soc Trop Med Hyg ISSN： 0035-9203 Impact factor: 2.184