Literature DB >> 6094835

Visualization of cAMP receptor protein-induced DNA kinking by electron microscopy.

A M Gronenborn, M V Nermut, P Eason, G M Clore.   

Abstract

The effect of specific DNA binding of the cAMP . cAMP receptor protein complex to two DNA fragments (301 and 2685 base-pairs in length) containing the lac operon has been investigated by electron microscopy. It is shown that specific DNA binding of the cAMP . cAMP receptor protein complex induces a kink of 30 to 45 degrees in the DNA with the apex of the kink located at the site of protein attachment. These findings lend direct visual support for the kinking hypothesis based on the observation of anomalous electrophoretic mobility of DNA fragments containing specifically bound cAMP receptor protein.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6094835     DOI： 10.1016/0022-2836(84)90166-9

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  7 in total

1.  Probing the sequence-specific interaction of the cyclic AMP receptor protein with DNA by site-directed mutagenesis.

Authors:  M E Gent; A M Gronenborn; R W Davies; G M Clore
Journal:  Biochem J       Date:  1987-03-15       Impact factor: 3.857

2.  A comparison of the DNA bending activities of the DNA binding proteins CRP and TFIID.

Authors:  K Gaston; A Bell; S Busby; M Fried
Journal:  Nucleic Acids Res       Date:  1992-07-11       Impact factor: 16.971

3.  The catabolite activator protein stabilizes its binding site in the E. coli lactose promoter.

Authors:  H DeGrazia; S Abhiraman; R M Wartell
Journal:  Nucleic Acids Res       Date:  1985-10-25       Impact factor: 16.971

4.  Binding and bending of the lambda replication origin by the phage O protein.

Authors:  K Zahn; F R Blattner
Journal:  EMBO J       Date:  1985-12-16       Impact factor: 11.598

5.  Visualization of RNA polymerase II ternary transcription complexes formed in vitro on a Xenopus laevis vitellogenin gene.

Authors:  B ten Heggeler; W Wahli
Journal:  EMBO J       Date:  1985-09       Impact factor: 11.598

6.  Variations of intramolecular ligation rates allow the detection of protein-induced bends in DNA.

Authors:  D Kotlarz; A Fritsch; H Buc
Journal:  EMBO J       Date:  1986-04       Impact factor: 11.598

7.  Escherichia coli integration host factor bends the DNA at the ends of IS1 and in an insertion hotspot with multiple IHF binding sites.

Authors:  P Prentki; M Chandler; D J Galas
Journal:  EMBO J       Date:  1987-08       Impact factor: 11.598
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.