Ultrastructural studies on human lymphoblastoid cells treated with n-butyrate and 12-O-tetradecanoylphorbol-13-acetate.

The effects of n-butyrate and/or 12-O-tetradecanoylphorbol-13-acetate (TPA) on Raji and P3HR-1 cells were examined using electron microscopy. n-Butyrate treatment caused a decrease in the number and elongation of surface microvilli. In addition, well-developed rough endoplasmic reticulum and a marked decrease in condensed chromatin clumps were observed in the nucleus. The filamentous structures and nuclear membrane-like structures, which were not reported previously, appeared occasionally in the nucleus. On the other hand, in the cells treated with TPA no marked morphological changes were observed except the higher cytoplasmic:nuclear ratio. n-Butyrate-induced morphological changes were not inhibited when combined with TPA or retinoic acid. Electron microscopic studies showed remarkable differences in morphological change in these cells treated with n-butyrate and/or TPA.