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Literature DB >> 6093046

Organization and expression of the E4 region of adenovirus 2.

Abstract

The E4 region of Adenovirus 2 is a leftward transcribed part of the viral genome. Its nucleotide sequence has already been analysed. From this sequence several open reading frames were defined, which could be used in the coding of the E4 proteins. Using S1 digestion of mRNA-DNA hybrids a precise mapping of donor and acceptor sites was done. Their use in various combinations allows the synthesis of mRNAs, able to direct the synthesis of at least 7 polypeptides, ranging in size from 9K to 34K. Comparison of the sequences of the different acceptor sites indicates that they all conform to the consensus sequence. Analysis of the ATG surrounding sequence shows that initiator ATG may be positively selected according to Kozak's rule.

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Year: 1984 PMID： 6093046 PMCID： PMC320191 DOI： 10.1093/nar/12.20.7649

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

22 in total

Organization and expression of the E4 region of adenovirus 2.

1. Complex splicing patterns of RNAs from the early regions of adenovirus-2.

2. Sequencing end-labeled DNA with base-specific chemical cleavages.

3. Mapping the DNA fragments produced by cleavage of EcoRI F fragment of adenovirus 2 with HaeIII, HpaII and AluI.

4. Regulation of adenovirus-2 gene expression at the level of transcriptional termination and RNA processing.

5. Nucleotide sequence of the EcoRI-F fragment of adenovirus 2 genome.

6. Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries.

7. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

8. Control of adenovirus early gene expression: a class of immediate early products.

9. Virus-receptor interaction in an adenovirus system.

10. Structure of the adenovirus 2 early mRNAs.

1. Sequence-specific trans-activation of the adenovirus EIIa early promoter by the viral EIV transcription unit.