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Literature DB >> 6092874

Genetic characterization of the Escherichia coli cyclopropane fatty acid (cfa) locus and neighboring loci.

Abstract

The phenotypically silent cyclopropane fatty acid synthesis (cfa) gene of Escherichia coli K-12 has been located on the genetic linkage map. This was accomplished by integrating (via homologous recombination) the selectable marker of a recombinant plasmid into the host chromosome near the cfa locus. This integration allowed the subsequent isolation of a cfa-linked transposon Tn10 insertion. Genetic mapping of the Tn10 insertion, using conventional techniques, placed the cfa locus at min 36.5 on the linkage map in the vicinity of several other non-selectable markers. We ordered cfa and these other loci by three-factor transductional analyses. Selection for excision of the Tn10 element resulted in several types of mutants which harbor mutations of cfa and of neighboring genes, presumably as a consequence of Tn10-catalyzed chromosomal rearrangements.

Entities: Chemical Gene Species

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Year: 1984 PMID： 6092874 DOI： 10.1007/bf00328074

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

22 in total

1. Inversions and deletions of the Salmonella chromosome generated by the translocatable tetracycline resistance element Tn10.

Authors: N Kleckner; K Reichardt; D Botstein
Journal: J Mol Biol Date: 1979-01-05 Impact factor: 5.469

2. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I.

Authors: M Monk; J Kinross
Journal: J Bacteriol Date: 1972-03 Impact factor: 3.490

Genetic characterization of the Escherichia coli cyclopropane fatty acid (cfa) locus and neighboring loci.

1. Inversions and deletions of the Salmonella chromosome generated by the translocatable tetracycline resistance element Tn10.

2. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I.

3. Selection and properties of Escherichia coli mutants defective in the synthesis of cyclopropane fatty acids.

4. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

5. Transductional mapping of ksgB and a new Tn5-induced kasugamycin resistance gene, ksgD, in Escherichia coli K-12.

Review 6. Linkage map of Escherichia coli K-12, edition 7.

7. Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies.

8. Two genetic loci for resistance to kasugamycin in Escherichia coli.

9. Physiological characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein.

10. Cloning and mapping of the manganese superoxide dismutase gene (sodA) of Escherichia coli K-12.

1. TonB interacts with nonreceptor proteins in the outer membrane of Escherichia coli.

Review 2. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Review 3. Linkage map of Escherichia coli K-12, edition 8.

4. Exonuclease III and the catalase hydroperoxidase II in Escherichia coli are both regulated by the katF gene product.

Review 5. Cyclopropane ring formation in membrane lipids of bacteria.

6. Superoxide and the production of oxidative DNA damage.

7. Characterization of Escherichia coli mutants completely defective in synthesis of cyclopropane fatty acids.

8. Submicromolar hydrogen peroxide disrupts the ability of Fur protein to control free-iron levels in Escherichia coli.

9. Role of the rfe gene in the synthesis of the O8 antigen in Escherichia coli K-12.