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Literature DB >> 6092854

Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase.

P Avila, F de la Cruz, E Ward, J Grinsted.

Abstract

In the presence of the Tn21 transposase, plasmids that contain a single Tn21 inverted repeat sequence fuse efficiently with other plasmids. This reaction occurs in recA strains, is independent of the transposon-encoded resolution system, and results in insertions into different sites in the recipient plasmid. All fusion products studied contained at least one complete copy of the donor plasmid; most also contained some duplication of it as well. The data are consistent with processive models of transposition.

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Year: 1984 PMID： 6092854 DOI： 10.1007/bf00332761

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

17 in total

9. Identification and characterization of Tn4653, a transposon covering the toluene transposon Tn4651 on TOL plasmid pWW0.

Authors: M Tsuda; T Iino
Journal: Mol Gen Genet Date: 1988-07

10. Novel beta-lactamase from Capnocytophaga sp.

Authors: J E Foweraker; P M Hawkey; J Heritage; H W Van Landuyt
Journal: Antimicrob Agents Chemother Date: 1990-08 Impact factor: 5.191

Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase.

1. Uniform nomenclature for bacterial plasmids: a proposal.

2. Replicon fusion mediated by a single-ended derivative of transposon Tn1721.

Review 3. Transposable elements in prokaryotes.

4. Rapid and efficient cosmid cloning.

5. Complementation of transposition of tnpA mutants of Tn3, Tn21, Tn501, and Tn1721.

6. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

7. DNA sequences of and complementation by the tnpR genes of Tn21, Tn501 and Tn1721.

8. Resolution of a hybrid cointegrate between transposons Tn501 and Tn1721 defines the recombination site.

9. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

10. Genetic and molecular characterization of Tn21, a multiple resistance transposon from R100.1.

1. DNA sequence of IS91 and identification of the transposase gene.

2. Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids.

3. Organization of complex transposon Tn2610 carrying two copies of tnpA and tnpR.

4. Effects of variation of inverted-repeat sequences on reactions mediated by the transposase of Tn21.

5. DNA sequence of the transposase gene of the class II transposon, Tn3926.

6. Transposition of Tn1000: activity of single or directly repeated termini.

7. Junction sequences generated by 'one-ended transposition'.

8. Analysis of the variable endpoints generated by one-ended transposition of Tn21.

9. Identification and characterization of Tn4653, a transposon covering the toluene transposon Tn4651 on TOL plasmid pWW0.

10. Novel beta-lactamase from Capnocytophaga sp.