| Literature DB >> 6091653 |
Y Nakatani, M Tsunoi, Y Hakeda, N Kurihara, K Fujita, M Kumegawa.
Abstract
The effect of parathyroid hormone (PTH) was tested on osteoblastic cells (clone MC3T3-E1) cultured in serum-free medium containing 0.3% bovine serum albumin. PTH stimulated an increase in cAMP production in a dose-related fashion up to a concentration of 0.5 unit/ml, the maximum increase being 23-fold above that of controls. Although PTH did not affect DNA content, this peptide also increased dose-dependently alkaline phosphatase activity up to a concentration of 0.5 unit/ml. PTH plus isobutylmethyl xanthine elevated enzyme activity 1.5-fold over that in PTH-treated cells. These results strongly indicate that PTH has a direct stimulatory effect on the differentiation of osteoblasts via intracellular cAMP production in vitro.Entities:
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Year: 1984 PMID： 6091653 DOI： 10.1016/s0006-291x(84)80219-3
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575