Decrease in ventricular beta-adrenergic receptors in trained diabetic rats.

The effects of physical training on beta-adrenergic receptors were evaluated in heart ventricular tissue of diabetic rats. Mild diabetes mellitus was induced in rats with streptozotocin (45 mg/kg, iv). They were then submitted to a progressive 10-week running programme on a treadmill. Binding studies were done at six different concentrations of (-) [3H]dihydroalprenolol (0.5 to 14.4 nM) with ventricular membrane preparations from control (n = 13), sedentary diabetic (n = 9) and trained diabetic rats (n = 10). Direct linear plot analysis of the data revealed that the total number of beta-adrenoceptors was reduced in sedentary diabetic rats as compared to control (2231 +/- 207 vs 2922 +/- 211 fmol/ventricles; P less than 0.05); however, there was no significant change in the receptor density expressed as fmol/mg of membrane protein (40 +/- 3 vs 43 +/- 3; P greater than 0.05). On the other hand, the beta-adrenergic binding sites were decreased in training diabetic rats, either expressed as the total number of receptors (1920 +/- 179 vs 2922 +/- 211; P less than 0.01), or as fmol/mg of membrane protein (30 +/- 3 vs 43 +/- 3; P less than 0.01). There was no significant change in the dissociation constant (KD) of these receptors between groups (KD = 4.08 +/- 0.51, 4.69 +/- 0.93 and 2.88 +/- 0.39 nM respectively for control, sedentary diabetic and diabetic trained animals). The basal epinephrine concentration was significantly increased in trained diabetic rats (102 +/- 21 pg/ml vs 47 +/- 7 for control (P less than 0.05) and vs 49 +/- 9 for sedentary diabetic (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)