Literature DB >> 6090567

Efficient production of hepatitis B surface antigen using a bovine papilloma virus-metallothionein vector.

N Hsiung, R Fitts, S Wilson, A Milne, D Hamer.   

Abstract

We have developed a highly efficient system for producing hepatitis B virus surface antigen in cultured mammalian cells. This system utilizes a recombinant bovine papilloma virus in which the hepatitis surface antigen coding sequences are inserted into the 5' untranslated region of the mouse metallothionein-I gene. Mouse fibroblasts stably transformed with this molecule produce surface antigen at levels as high as 10 mg/L/24 h and can be maintained in continuous culture for up to 85 days.

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Year:  1984        PMID： 6090567

Source DB:  PubMed          Journal:  J Mol Appl Genet        ISSN： 0271-6801


  12 in total

1.  Inactivation of recombinant plasmid DNA from a human erythropoietin-producing mouse cell line grown on a large scale.

Authors:  M R Fibi; M Bröker; R Schulz; R Johannsen; G Zettlmeissl
Journal:  Appl Microbiol Biotechnol       Date:  1991-08       Impact factor: 4.813

Review 2.  [Warts and viruses].

Authors:  U Linz
Journal:  Naturwissenschaften       Date:  1988-05

Review 3.  The bovine papillomavirus genome and its uses as a eukaryotic vector.

Authors:  P E Stephens; C C Hentschel
Journal:  Biochem J       Date:  1987-11-15       Impact factor: 3.857

4.  Expression of hepatitis B virus surface and core antigens: influences of pre-S and precore sequences.

Authors:  A McLachlan; D R Milich; A K Raney; M G Riggs; J L Hughes; J Sorge; F V Chisari
Journal:  J Virol       Date:  1987-03       Impact factor: 5.103

5.  Use of a hybrid vaccinia virus-T7 RNA polymerase system for expression of target genes.

Authors:  T R Fuerst; P L Earl; B Moss
Journal:  Mol Cell Biol       Date:  1987-07       Impact factor: 4.272

6.  The effect of protein synthesis inhibitors on the glycosylation site occupancy of recombinant human prolactin.

Authors:  M Shelikoff; A J Sinskey; G Stephanopoulos
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

7.  Expression of the hepatitis B virus surface antigen in Drosophila S2 cells.

Authors:  Soraia A C Jorge; Alexandra S Santos; Angela Spina; Carlos A Pereira
Journal:  Cytotechnology       Date:  2008-07-02       Impact factor: 2.058

8.  Expression of the human papillomavirus type 18 E7 gene by a cassette-vector system for the transcription and translation of open reading frames in eukaryotic cells.

Authors:  H U Bernard; T Oltersdorf; K Seedorf
Journal:  EMBO J       Date:  1987-01       Impact factor: 11.598

9.  Lines of BPV-transformed murine cells that constitutively express influenza virus hemagglutinin.

Authors:  J Sambrook; L Rodgers; J White; M J Gething
Journal:  EMBO J       Date:  1985-01       Impact factor: 11.598

10.  SV40 T antigen and the exocytotic pathway.

Authors:  S Sharma; L Rodgers; J Brandsma; M J Gething; J Sambrook
Journal:  EMBO J       Date:  1985-06       Impact factor: 11.598
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