| Literature DB >> 6090387 |
S Clancy, C Mann, R W Davis, M P Calos.
Abstract
Saccharomyces cerevisiae was transformed with DNA by the lithium acetate method. Mutation of nonselected markers on the transforming vector was observed at a frequency several orders of magnitude higher than spontaneous mutation frequencies. These mutations were shown to be deletions. Linearization of the vector before transformation stimulated deletion formation.Entities:
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Year: 1984 PMID: 6090387 PMCID: PMC215771 DOI: 10.1128/jb.159.3.1065-1067.1984
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490