Literature DB >> 6089625

Enzyme-linked immunosorbent assay for detection of transmissible gastroenteritis virus antibody in swine sera.

L D Nelson, C L Kelling.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for detection and quantification of serum antibodies to transmissible gastroenteritis virus (TGEV) in swine. Sera from pigs inoculated with cell culture-origin TGEV or gut-origin TGEV were tested for anti-TGEV antibody by ELISA and by serum virus-neutralization test (NT). The ELISA detected antibody 3 days (av) sooner than did the NT when sera from pigs inoculated with cell culture-origin TGEV were tested and 1 day sooner than did the NT when sera from pigs inoculated with gut-origin TGEV were tested. The ELISA appeared to be more sensitive than the NT, since ELISA was more responsive to low-level antibody and ELISA titers exceeded NT titers.

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Year:  1984        PMID： 6089625

Source DB:  PubMed          Journal:  Am J Vet Res        ISSN： 0002-9645            Impact factor:   1.156


  3 in total

1.  Evaluation of an enzyme-linked immunosorbent assay for the detection of transmissible gastroenteritis virus antibodies.

Authors:  T Hohdatsu; Y Eiguchi; S Ide; K Baba; H Yamagishi; T Kume; M Matumoto
Journal:  Vet Microbiol       Date:  1987-01       Impact factor: 3.293

2.  Isolation of peplomer glycoprotein E2 of transmissible gastroenteritis virus and application in enzyme-linked immunosorbent assay.

Authors:  G G Rukhadze; T I Aliper; V A Sergeev
Journal:  J Clin Microbiol       Date:  1989-08       Impact factor: 5.948

3.  An ELISA for the detection of serum antibodies to both transmissible gastroenteritis virus and porcine respiratory coronavirus.

Authors:  D J Paton; I H Brown; E K Vaz
Journal:  Br Vet J       Date:  1991 Jul-Aug
  3 in total

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