Literature DB >> 6088798

Monoclonal antibody to simian virus 40 small t.

X Montano, D P Lane.   

Abstract

A monoclonal antibody, PAb280, was produced that recognizes simian virus 40 (SV40) small t but does not react with SV40 large T. The specificity of the antibody was analyzed by immunoprecipitation of labeled cell extracts, Western blotting, and immunocytochemistry. Small t was found to accumulate late in the SV40 lytic cycle and was localized in both the cytoplasm and the nucleus of cells infected with wild-type SV40. Importantly, antibodies against determinants common to SV40 large T and small t did not appear to be able to recognize the cytoplasmic form of SV40 small t at the immunocytochemical level. The localization of small t within the nucleus appeared to be distinct from that of large T.

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Year:  1984        PMID： 6088798      PMCID： PMC255842     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  29 in total

1.  Processing and expression of early SV40 mRNA: a role for RNA conformation in splicing.

Authors:  G Khoury; P Gruss; R Dhar; C J Lai
Journal:  Cell       Date:  1979-09       Impact factor: 41.582

2.  Effect of alkylation on the physical properties of simian virus 40 T-antigen species.

Authors:  L V Crawford; P Z O'Farrell
Journal:  J Virol       Date:  1979-02       Impact factor: 5.103

3.  Mutants of SV40 with an altered small t protein are reduced in their ability to transform cells.

Authors:  M J Sleigh; W C Topp; R Hanich; J F Sambrook
Journal:  Cell       Date:  1978-05       Impact factor: 41.582

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  An immunochemical investigation of SV40 T-antigens. 2. Quantitation of antigens and antibody activities.

Authors:  L V Crawford; D C Pim; D P Lane
Journal:  Virology       Date:  1980-01-30       Impact factor: 3.616

7.  Hybrid plasmacytoma production: fusions with adult spleen cells, monoclonal spleen fragments, neonatal spleen cells and human spleen cells.

Authors:  R H Kennett; K A Denis; A S Tung; N R Klinman
Journal:  Curr Top Microbiol Immunol       Date:  1978       Impact factor: 4.291

8.  An immunochemical investigation of SV40 T antigens. 1. Production properties and specificity of rabbit antibody to purified simian virus 40 large-T antigen.

Authors:  D P Lane; A K Robbins
Journal:  Virology       Date:  1978-06-01       Impact factor: 3.616

9.  Simian virus 40 small t antigen is not required for the maintenance of transformation but may act as a promoter (cocarcinogen) during establishment of transformation in resting rat cells.

Authors:  R Seif; R G Martin
Journal:  J Virol       Date:  1979-12       Impact factor: 5.103

10.  An antibody to a synthetic peptide that recognises SV40 small-t antigen.

Authors:  R Harvey; R Faulkes; P Gillett; N Lindsay; E Paucha; A Bradbury; A E Smith
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
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  13 in total

Review 1.  Protein phosphatases and DNA tumor viruses: transformation through the back door?

Authors:  M C Mumby; G Walter
Journal:  Cell Regul       Date:  1991-08

2.  MDM2 is a target of simian virus 40 in cellular transformation and during lytic infection.

Authors:  W Henning; G Rohaly; T Kolzau; U Knippschild; H Maacke; W Deppert
Journal:  J Virol       Date:  1997-10       Impact factor: 5.103

3.  Monoclonal antibody analysis of p53 expression in normal and transformed cells.

Authors:  J W Yewdell; J V Gannon; D P Lane
Journal:  J Virol       Date:  1986-08       Impact factor: 5.103

4.  Cellular proteins which can specifically associate with simian virus 40 small t antigen.

Authors:  C I Murphy; I Bikel; D M Livingston
Journal:  J Virol       Date:  1986-09       Impact factor: 5.103

5.  The t-unique coding domain is important to the transformation maintenance function of the simian virus 40 small t antigen.

Authors:  I Bikel; H Mamon; E L Brown; J Boltax; M Agha; D M Livingston
Journal:  Mol Cell Biol       Date:  1986-04       Impact factor: 4.272

6.  Characterization of a 31 kDa polypeptide that accumulates in the light-harvesting apparatus of maize leaves during chilling.

Authors:  D B Hayden; P S Covello; N R Baker
Journal:  Photosynth Res       Date:  1988-03       Impact factor: 3.573

7.  Modifications to Thylakoid Composition during Development of Maize Leaves at Low Growth Temperatures.

Authors:  G Y Nie; N R Baker
Journal:  Plant Physiol       Date:  1991-01       Impact factor: 8.340

8.  Mutations in the phosphorylation sites of simian virus 40 (SV40) T antigen alter its origin DNA-binding specificity for sites I or II and affect SV40 DNA replication activity.

Authors:  J Schneider; E Fanning
Journal:  J Virol       Date:  1988-05       Impact factor: 5.103

9.  Serotonin uptake, storage, and synthesis in an immortalized committed cell line derived from mouse teratocarcinoma.

Authors:  M H Buc-Caron; J M Launay; D Lamblin; O Kellermann
Journal:  Proc Natl Acad Sci U S A       Date:  1990-03       Impact factor: 11.205

10.  Multiple, distinct trans-activation functions are encoded by the simian virus 40 large T and small t antigens, only some of which require the 82-residue amino-terminal common domain.

Authors:  M R Loeken
Journal:  J Virol       Date:  1993-12       Impact factor: 5.103
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