Literature DB >> 6088638

Reverse enzyme-linked immunospot assay (RELISPOT) for the detection of cells secreting immunoreactive substances.

C C Czerkinsky, A Tarkowski, L A Nilsson, O Ouchterlony, H Nygren, C Gretzer.   

Abstract

A reverse modification of the recently described enzyme-linked immunospot assay (ELISPOT), based on localized enzyme-substrate reactions in gel, is described for the enumeration of antigen-secreting cells using petri dishes coated with specific antibodies. As a model the detection of mouse and human immunoglobulin-secreting cells has been evaluated. Simple and sensitive, this new method, termed RELISPOT, can be adapted for the quantitation of secreted antigen thus providing additional information on the metabolic state of the population of cells tested.

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Year:  1984        PMID： 6088638     DOI： 10.1016/0022-1759(84)90017-6

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  16 in total

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3.  Enumeration of IgA producing cells by the enzyme linked immunospot (ELISPOT) technique to evaluate sulphasalazine effects in inflammatory arthritides.

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4.  Optimal blood mononuclear cell isolation procedures for gamma interferon enzyme-linked immunospot testing of healthy Swedish and Tanzanian subjects.

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Authors:  A Tarkowski; R Holmdahl; K Rubin; L Klareskog; L A Nilsson; K Gunnarsson
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8.  IgA antibody-producing cells in peripheral blood after antigen ingestion: evidence for a common mucosal immune system in humans.

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9.  Simultaneous induction of rheumatoid factor- and antigen-specific antibody-secreting cells during the secondary immune response in man.

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10.  Features of renal vasculitis in autoimmune MRL lpr/lpr mice: phenotypes and functional properties of infiltrating cells.

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