Literature DB >> 6086927

Blot hybridisation analysis of genomic DNA.

S Vandenplas, I Wiid, A Grobler-Rabie, K Brebner, M Ricketts, G Wållis, A Bester, C Boyd, C Måthew.   

Abstract

Restriction endonuclease analysis of specific gene sequences is proving to be a valuable technique for characterisation and diagnosis of inherited disorders. This paper describes detailed protocols for isolation, restriction, and blot hybridisation of genomic DNA. Problems and alternatives in the procedure are discussed and a troubleshooting guide has been provided to help rectify faults.

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Year:  1984        PMID： 6086927      PMCID： PMC1049258          DOI： 10.1136/jmg.21.3.164

Source DB:  PubMed          Journal:  J Med Genet        ISSN： 0022-2593            Impact factor:   6.318


  17 in total

1.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

2.  Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.

Authors:  R A Laskey; A D Mills
Journal:  FEBS Lett       Date:  1977-10-15       Impact factor: 4.124

3.  Antenatal diagnosis of sickle-cell anaemia by D.N.A. analysis of amniotic-fluid cells.

Authors:  Y W Kan; A M Dozy
Journal:  Lancet       Date:  1978-10-28       Impact factor: 79.321

4.  Model for antenatal diagnosis of beta-thalassaemia and other monogenic disorders by molecular analysis of linked DNA polymorphisms.

Authors:  P F Little; G Annison; S Darling; R Williamson; L Camba; B Modell
Journal:  Nature       Date:  1980-05-15       Impact factor: 49.962

Review 5.  The application of DNA recombinant technology to the analysis of the human genome and genetic disease.

Authors:  K E Davies
Journal:  Hum Genet       Date:  1981       Impact factor: 4.132

6.  A physical map of the DNA regions flanking the rabbit beta-globin gene.

Authors:  A J Jeffreys; R A Flavell
Journal:  Cell       Date:  1977-10       Impact factor: 41.582

7.  Polymorphism of DNA sequence adjacent to human beta-globin structural gene: relationship to sickle mutation.

Authors:  Y W Kan; A M Dozy
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205

8.  Release of infectious Epstein-Barr virus by transformed marmoset leukocytes.

Authors:  G Miller; M Lipman
Journal:  Proc Natl Acad Sci U S A       Date:  1973-01       Impact factor: 11.205

Review 9.  Construction of a genetic linkage map in man using restriction fragment length polymorphisms.

Authors:  D Botstein; R L White; M Skolnick; R W Davis
Journal:  Am J Hum Genet       Date:  1980-05       Impact factor: 11.025

10.  Cloning a cDNA for the pro-alpha 2 chain of human type I collagen.

Authors:  J C Myers; M L Chu; S H Faro; W J Clark; D J Prockop; F Ramirez
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205
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  63 in total

1.  Exclusion map of Salla disease: attempts to localize the disease gene using a computer program.

Authors:  L Haataja; J Schleutker; M Renlund; A Palotie; L Peltonen; P Aula
Journal:  Hum Genet       Date:  1992-01       Impact factor: 4.132

2.  The distribution of the HLA-DQ alpha alleles and genotypes in the Finnish population as determined by the use of DNA amplification and allele specific oligonucleotides.

Authors:  A Sajantila; M Ström; B Budowle; P J Tienari; C Ehnholm; L Peltonen
Journal:  Int J Legal Med       Date:  1991       Impact factor: 2.686

3.  Marfan syndrome: no evidence for heterogeneity in different populations, and more precise mapping of the gene.

Authors:  K Kainulainen; B Steinmann; F Collins; H C Dietz; C A Francomano; A Child; M W Kilpatrick; D J Brock; M Keston; R E Pyeritz
Journal:  Am J Hum Genet       Date:  1991-09       Impact factor: 11.025

4.  RFLP analysis of three different types of acute intermittent porphyria.

Authors:  R Kauppinen; L Peltonen; A Palotie; P Mustajoki
Journal:  Hum Genet       Date:  1990-07       Impact factor: 4.132

5.  Two new polymorphic markers in the human pro alpha 2(1) collagen gene.

Authors:  D K Brebner; A F Grobler-Rabie; A J Bester; C G Mathew; C D Boyd
Journal:  Hum Genet       Date:  1985       Impact factor: 4.132

6.  Gene defect in hypodontia: exclusion of MSX1 and MSX2 as candidate genes.

Authors:  P Nieminen; S Arte; S Pirinen; L Peltonen; I Thesleff
Journal:  Hum Genet       Date:  1995-09       Impact factor: 4.132

7.  Osteogenesis imperfecta type III: mutations in the type I collagen structural genes, COL1A1 and COL1A2, are not necessarily responsible.

Authors:  G A Wallis; B Sykes; P H Byers; C G Mathew; D Viljoen; P Beighton
Journal:  J Med Genet       Date:  1993-06       Impact factor: 6.318

8.  The genetic locus for free sialic acid storage disease maps to the long arm of chromosome 6.

Authors:  L Haataja; J Schleutker; A P Laine; M Renlund; M L Savontaus; C Dib; J Weissenbach; L Peltonen; P Aula
Journal:  Am J Hum Genet       Date:  1994-06       Impact factor: 11.025

9.  Genetic variation in apolipoprotein D and Alzheimer's disease.

Authors:  Seppo Helisalmi; Mikko Hiltunen; Saila Vepsäläinen; Susan Iivonen; Elizabeth H Corder; Maarit Lehtovirta; Arto Mannermaa; Anne Maria Koivisto; Hilkka Soininen
Journal:  J Neurol       Date:  2004-08       Impact factor: 4.849

10.  Proving paternity of children with deceased fathers.

Authors:  P Helminen; V Johnsson; C Ehnholm; L Peltonen
Journal:  Hum Genet       Date:  1991-10       Impact factor: 4.132
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