Human lymphoblastoid cell lines established from peripheral blood lymphocytes secreting immunoglobulins directed against herpes simplex virus.

Peripheral blood lymphocytes (PBL) from genital herpes simplex virus type 2 (HSV-2) patients were transformed with Epstein-Barr virus. Using conditions optimized for successful transformation, two lymphoblastoid cell lines (LCL) have been established, which secrete immunoglobulin G (IgG) to HSV. These results suggest that PBL from patients suffering recurrent herpes virus infections can be used to establish LCL secreting HSV antigen specific human immunoglobulins.