Receptor mediated pathways for interferon action: in vivo implications.

High affinity receptors for human interferon (IFN) are a common surface component of human nucleated cells. A model culture system has been used to mark the activation of surface receptors by IFN as the first step in several, possibly separate, pathways of action, some of which depend on the continued presence of IFN. Two types of equilibrium binding can be seen: an initial one of short duration corresponding to receptor activation and a second of longer duration corresponding to activation of a second site by IFN. Various different responses to IFN are seen to be determined by one or other of these equilibria. In vivo, implications are discussed in terms of the pharmacokinetic distribution of IFN, with particular reference to intravenous injection. Desensitization of target cells with respect to late equilibrium effects is seen as a possible consequence of receptor activation against the background of changing IFN concentration in vivo. A modulating effect of combined IFN species is proposed.