Literature DB >> 6085559

Separation and subfractionation of small numbers of cells (approximately 10(6)) by countercurrent distribution in dextran-poly(ethylene glycol) aqueous-phase systems.

H Walter, E J Krob.   

Abstract

Separation and subfractionation of cells on the basis of subtle differences in surface properties by partitioning in dextran-poly(ethylene glycol) aqueous phase systems is an established method. We report here that the incorporation of fetal bovine serum into such systems permits countercurrent distribution of small quantities of cells (approximately 10(6]. In the absence of serum such small quantities of cells are lost (probably by adherence) and cannot be recovered after countercurrent distribution.

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Year:  1984        PMID： 6085559     DOI： 10.1007/bf02788631

Source DB:  PubMed          Journal:  Cell Biophys        ISSN： 0163-4992


  10 in total

1.  Counter-current distribution of cells.

Authors:  P A ALBERTSSON; G D BAIRD
Journal:  Exp Cell Res       Date:  1962-11       Impact factor: 3.905

2.  The removal of leukocytes and platelets from whole blood.

Authors:  E Beutler; C West; K G Blume
Journal:  J Lab Clin Med       Date:  1976-08

3.  Subfractionation of human peripheral blood lymphocytes on the basis of their surface properties by partitioning in two-polymer aqueous phase systems.

Authors:  H Walter; T J Webber; J P Michalski; C C McCombs; B J Moncla; E J Krob; L L Graham
Journal:  J Immunol       Date:  1979-10       Impact factor: 5.422

4.  Influence of temperature and method of centrifugation on the separation of erythrocytes.

Authors:  J R Murphy
Journal:  J Lab Clin Med       Date:  1973-08

5.  Electromotive phenomena in partition of erythrocytes in aqueous polymer two phase systems.

Authors:  R Reitherman; S D Flanagan; S H Barondes
Journal:  Biochim Biophys Acta       Date:  1973-02-28

6.  The nature of the cell membrane charge measured by partition in aqueous two-polymer phase systems: differentiation of classes of beef erythrocytes.

Authors:  H Walter; R Tung; L J Jackson; G V Seaman
Journal:  Biochem Biophys Res Commun       Date:  1972-08-07       Impact factor: 3.575

7.  Aging of erythrocytes results in altered red cell surface properties in the rat, but not in the human. Studies by partitioning in two-polymer aqueous phase systems.

Authors:  H Walter; E J Krob; G S Ascher
Journal:  Biochim Biophys Acta       Date:  1981-02-20

8.  Subfractionation of malignant variants of metastatic murine lymphosarcoma cells by countercurrent distribution in two-polymer aqueous phases.

Authors:  K M Miner; H Walter; G L Nicolson
Journal:  Biochemistry       Date:  1981-10-13       Impact factor: 3.162

9.  Detection of surface differences between two closely related cell populations by partitioning isotopically labeled mixed cell populations in two-polymer aqueous phases. I. Human red blood cell subpopulations.

Authors:  H Walter; E J Krob
Journal:  Cell Biophys       Date:  1983-09

10.  Correlation between phagocytic and membrane surface properties reflected by partitioning of human peripheral blood monocytes in two-polymer aqueous phases.

Authors:  H Walter; L L Graham; E J Krob; M Hill
Journal:  Biochim Biophys Acta       Date:  1980-11-04
  10 in total
  1 in total

1.  Neurocognitive signatures of phonemic sequencing in expert backward speakers.

Authors:  María José Torres-Prioris; Diana López-Barroso; Estela Càmara; Sol Fittipaldi; Lucas Sedeño; Agustín Ibáñez; Marcelo L Berthier; Adolfo M García
Journal:  Sci Rep       Date:  2020-06-30       Impact factor: 4.379
  1 in total

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