Literature DB >> 6084988

Immunoblotting procedure for the analysis of electrophoretically-fractionated bacterial lipopolysaccharide.

S Sturm, P Fortnagel, K N Timmis.   

Abstract

A procedure is described for the efficient transfer of fractionated bacterial lipopolysaccharide (LPS) from SDS-polyacrylamide gels to nitrocellulose filters, and its subsequent display by a peroxidase-linked antibody. The method is sensitive, and reveals and resolves high molecular weight LPS molecules having side chain lengths of up to and greater than 30 repeat units. It is useful for the rapid analysis of LPS in bacterial outer membrane preparations.

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Year:  1984        PMID： 6084988     DOI： 10.1007/bf00454926

Source DB:  PubMed          Journal:  Arch Microbiol        ISSN： 0302-8933            Impact factor:   2.552


  17 in total

1.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

Authors:  M J Osborn; J E Gander; E Parisi; J Carson
Journal:  J Biol Chem       Date:  1972-06-25       Impact factor: 5.157

3.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors:  G Fairbanks; T L Steck; D F Wallach
Journal:  Biochemistry       Date:  1971-06-22       Impact factor: 3.162

4.  Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2.

Authors:  R C Goldman; L Leive
Journal:  Eur J Biochem       Date:  1980

5.  Immunochemical characterization of Neisseria meningitidis serotype antigens by immunodiffusion and SDS-polyacrylamide gel electrophoresis immunoperoxidase techniques and the distribution of serotypes among cases and carriers.

Authors:  J T Poolman; C T Hopman; H C Zanen
Journal:  J Gen Microbiol       Date:  1980-02

6.  Electrophoretic transfer of viral proteins to nitrocellulose sheets and detection with peroxidase-bound lectins and protein A.

Authors:  P Ramírez; J A Bonilla; E Moreno; P León
Journal:  J Immunol Methods       Date:  1983-08-12       Impact factor: 2.303

7.  A dot-immunobinding assay for monoclonal and other antibodies.

Authors:  R Hawkes; E Niday; J Gordon
Journal:  Anal Biochem       Date:  1982-01-01       Impact factor: 3.365

8.  Small virulence plasmid of Shigella dysenteriae 1 strain W30864 encodes a 41,000-dalton protein involved in formation of specific lipopolysaccharide side chains of serotype 1 isolates.

Authors:  H Watanabe; A Nakamura; K N Timmis
Journal:  Infect Immun       Date:  1984-10       Impact factor: 3.441

9.  Monoclonal antibodies against Neisseria meningitidis lipopolysaccharide.

Authors:  R J Sugasawara; C Prato; J E Sippel
Journal:  Infect Immun       Date:  1983-12       Impact factor: 3.441

10.  A small plasmid in Shigella dysenteriae 1 specifies one or more functions essential for O antigen production and bacterial virulence.

Authors:  H Watanabe; K N Timmis
Journal:  Infect Immun       Date:  1984-01       Impact factor: 3.441
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  5 in total

1.  Immunochemical characterization of two surface polysaccharides of Bacteroides fragilis.

Authors:  A Pantosti; A O Tzianabos; A B Onderdonk; D L Kasper
Journal:  Infect Immun       Date:  1991-06       Impact factor: 3.441

2.  A Rhizobium meliloti lipopolysaccharide mutant altered in competitiveness for nodulation of alfalfa.

Authors:  A Lagares; G Caetano-Anollés; K Niehaus; J Lorenzen; H D Ljunggren; A Pühler; G Favelukes
Journal:  J Bacteriol       Date:  1992-09       Impact factor: 3.490

3.  In vitro and in vivo activity of polyclonal and monoclonal human immunoglobulins G, M, and A against Pseudomonas aeruginosa lipopolysaccharide.

Authors:  G B Pier; D Thomas; G Small; A Siadak; H Zweerink
Journal:  Infect Immun       Date:  1989-01       Impact factor: 3.441

4.  Zinc Deficiency During Pregnancy Leads to Altered Microbiome and Elevated Inflammatory Markers in Mice.

Authors:  Ann Katrin Sauer; Andreas M Grabrucker
Journal:  Front Neurosci       Date:  2019-11-29       Impact factor: 4.677

Review 5.  Immunoblotting and dot blotting.

Authors:  D I Stott
Journal:  J Immunol Methods       Date:  1989-05-12       Impact factor: 2.303
  5 in total

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