The characterization and uses of monoclonal antibodies to respiratory syncytial virus.

Fifteen hybridomas secreting monoclonal antibodies against respiratory syncytial virus (RSV) have been produced. Three react with nucleoprotein (MW 42 000 daltons), 1 with phosphoprotein (MW 36 000 daltons), 1 with a larger protein (MW 60 000 daltons) and 10 with the fusion glycoprotein (MW 46 000 + 22 000 daltons). By immunofluorescent staining of infected cells, 5 monoclonal antibodies bind to cytoplasmic inclusions and the remainder give diffuse cytoplasmic fluorescence. Virus infectivity is neutralized by two monoclonal antibodies. Thirteen react with both bovine and human strains of RSV, two fail to react with three human strains. The antibodies have been used to induce passive protection in mice against challenge with RSV and to purify viral components on immunoadsorbent columns.