Analysis of a serological determinant of H-Y antigen: evidence for carbohydrate specificity using an H-Y specific monoclonal antibody.

Using a monoclonal antibody to H-Y antigen we characterized the molecular determinant responsible for H-Y reactivity. H-Y-positive cells were treated with enzymes which alter carbohydrate structure and then were tested for their ability to absorb monoclonal anti-H-Y antibody. Indications are that the serological determinant recognized by this antibody is a glycoconjugate containing terminal non-reducing and internal galactosyl residues.