Literature DB >> 606258

Affinity chromatography of thiol-containing purines and ribonucleic acid.

W T Melvin, H M Keir.   

Abstract

Evidence is presented for the specific interaction of 6-mercaptopurine with mercurated cellulose. Following from this, a new method is described for the affinity chromatography of thiol-containing molecules and of RNA containing incorporated 6-thioguanosine on columns of mercurated cellulose. This technique may find application in the study of RNA metabolism and gene expression.

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Year:  1977        PMID： 606258      PMCID： PMC1183813          DOI： 10.1042/bj1680595

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  7 in total

1.  Mercurated polynucleotides: new probes for hybridization and selective polymer fractionation.

Authors:  R M Dale; D C Ward
Journal:  Biochemistry       Date:  1975-06-03       Impact factor: 3.162

2.  Deoxyribonucleic acid polymerases of BHK-21/C13 cells. Partial purification and characterization of the enzymes.

Authors:  R K Craig; H M Keir
Journal:  Biochem J       Date:  1975-02       Impact factor: 3.857

3.  RNA aggregation during sulfhydryl-agarose chromatography of mercurated RNA.

Authors:  D A Konkel; V M Ingram
Journal:  Nucleic Acids Res       Date:  1977-06       Impact factor: 16.971

4.  A cellulose mercurial: preparation, properties and use in antibody purification.

Authors:  J R Shainoff
Journal:  J Immunol       Date:  1968-01       Impact factor: 5.422

5.  A biochemical mechanism for the delayed cytotoxic reaction of 6-mercaptopurine.

Authors:  D M Tidd; A R Paterson
Journal:  Cancer Res       Date:  1974-04       Impact factor: 12.701

6.  Fidelity of chromatin transcription in vitro.

Authors:  H Biessmann; R A Gjerset; B Levy; B J McCarthy
Journal:  Biochemistry       Date:  1976-10-05       Impact factor: 3.162

7.  The synthesis and enzymatic polymerization of nucleotides containing mercury: potential tools for nucleic acid sequencing and structural analysis.

Authors:  R M Dale; D C Livingston; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1973-08       Impact factor: 11.205
  7 in total
  2 in total

1.  RNA polymerase II elongation complex. Elongation complexes purified using an anti-RNA antibody do not contain initiation factor alpha.

Authors:  D Reines
Journal:  J Biol Chem       Date:  1991-06-05       Impact factor: 5.157

2.  Onset of ribosome degradation during cessation of growth in BHK-21/C13 cells.

Authors:  W T Melvin; H M Keir
Journal:  Biochem J       Date:  1978-12-15       Impact factor: 3.857
  2 in total

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