| Literature DB >> 6062 |
J B Bell, J P Gelugne, K B Jacobson.
Abstract
The inhibitory effect of tRNA on yeast 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase (EC 4.1.2.15) has been reinvestigated. From earlier studies the inhibition by tRNAPhe appeared to be quite specific. This study shows that tRNAPhe is indeed a potent inhibitor but so is unfractionated tRNA, as well as ribosomal RNA and heparin. Complete digestion to mononucleotides relieves the inhibition. Since the enzyme requires a metal ion (Co2+) we suggest that the RNA and heparin are inhibitory by virtue of their capacity to chelate the Co2+.Entities:
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Year: 1976 PMID： 6062 DOI： 10.1016/0005-2787(76)90187-8
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002