Literature DB >> 6058112

The microbial oxidation of methanol. Purification and properties of the alcohol dehydrogenase of Pseudomonas sp. M27.

C Anthony, L J Zatman.   

Abstract

1. A method for the purification of the nicotinamide nucleotide-independent alcohol dehydrogenase of Pseudomonas sp. M27 is described. 2. In the analytical ultracentrifuge, the purified enzyme shows a single major component of molecular weight 146000. 3. On electrophoresis in polyacrylamide gels between pH5.0 and 9.55, it shows only one protein band and the isoelectric point appears to be between pH7.0 and 8.0. 4. Spectrographic analysis indicates no significant metal content. 5. Amino acid analysis shows an unusually small number of cysteine/cystine residues per molecule as well as about 4.1% of glucosamine. 6. The role of ammonia as enzyme activator has been investigated.

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Year:  1967        PMID： 6058112      PMCID： PMC1271237          DOI： 10.1042/bj1040953

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  8 in total

1.  Ultracentrifugal studies of rat, rabbit and guinea-pig serum albumins.

Authors:  P A CHARLWOOD
Journal:  Biochem J       Date:  1961-01       Impact factor: 3.857

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Ultraviolet absorption spectra of proteins and amino acids.

Authors:  G H BEAVEN; E R HOLIDAY
Journal:  Adv Protein Chem       Date:  1952

4.  The microbial oxidation of methanol. The prosthetic group of the alcohol dehydrogenase of Pseudomonas sp. M27: a new oxidoreductase prosthetic group.

Authors:  C Anthony; L J Zatman
Journal:  Biochem J       Date:  1967-09       Impact factor: 3.857

5.  The microbial oxidation of methanol. 2. The methanol-oxidizing enzyme of Pseudomonas sp. M 27.

Authors:  C Anthony; L J Zatman
Journal:  Biochem J       Date:  1964-09       Impact factor: 3.857

6.  The microbial oxidation of methanol. 1. Isolation and properties of Pseudomonas sp. M27.

Authors:  C Anthony; L J Zatman
Journal:  Biochem J       Date:  1964-09       Impact factor: 3.857

7.  The microbial oxidation of methanol. The alcohol dehydrogenase of Pseudomonas sp. M27.

Authors:  C Anthony; L J Zatman
Journal:  Biochem J       Date:  1965-09       Impact factor: 3.857

8.  Estimation of the molecular weights of proteins by Sephadex gel-filtration.

Authors:  P Andrews
Journal:  Biochem J       Date:  1964-05       Impact factor: 3.766
  8 in total
  47 in total

1.  Oxidation of organic C1 compounds by Hyphomicrobium spp.

Authors:  W Harder; M M Attwood
Journal:  Antonie Van Leeuwenhoek       Date:  1975       Impact factor: 2.271

2.  Structural aspects of the dye-linked alcohol dehydrogenase of Rhodopseudomonas acidophila.

Authors:  C W Bamforth; J R Quayle
Journal:  Biochem J       Date:  1979-09-01       Impact factor: 3.857

3.  The interaction of methanol dehydrogenase and cytochrome cL in the acidophilic methylotroph Acetobacter methanolicus.

Authors:  H T Chan; C Anthony
Journal:  Biochem J       Date:  1991-11-15       Impact factor: 3.857

4.  The microbial metabolism of C1 compounds. The cytochromes of Pseudomaonas AM1.

Authors:  C Anthony
Journal:  Biochem J       Date:  1975-02       Impact factor: 3.857

5.  Studies on methanol - oxidizing yeast. III. Enzyme.

Authors:  O Volfová
Journal:  Folia Microbiol (Praha)       Date:  1975       Impact factor: 2.099

6.  The microbial oxidation of methanol. The prosthetic group of the alcohol dehydrogenase of Pseudomonas sp. M27: a new oxidoreductase prosthetic group.

Authors:  C Anthony; L J Zatman
Journal:  Biochem J       Date:  1967-09       Impact factor: 3.857

7.  Production of pyrroloquinoline quinone by using methanol-utilizing bacteria.

Authors:  T Urakami; K Yashima; H Kobayashi; A Yoshida; C Ito-Yoshida
Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792

8.  Synthesis of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) from Methanol and n-Amyl Alcohol by the Methylotrophic Bacteria Paracoccus denitrificans and Methylobacterium extorquens.

Authors:  S Ueda; S Matsumoto; A Takagi; T Yamane
Journal:  Appl Environ Microbiol       Date:  1992-11       Impact factor: 4.792

9.  Cloning of the genes involved in synthesis of coenzyme pyrrolo-quinoline-quinone from Acinetobacter calcoaceticus.

Authors:  N Goosen; D A Vermaas; P van de Putte
Journal:  J Bacteriol       Date:  1987-01       Impact factor: 3.490

10.  Replacement of enzyme-bound calcium with strontium alters the kinetic properties of methanol dehydrogenase.

Authors:  T K Harris; V L Davidson
Journal:  Biochem J       Date:  1994-05-15       Impact factor: 3.857
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