Literature DB >> 6056633

Purification of an acid alpha-glucosidase by dextran-gel filtration.

F Auricchio, C B Bruni.   

Abstract

1. The behaviour of rat liver alpha-glucosidases on dextran gel (Sephadex G-100) columns was studied. A ;retardation' of an acid alpha-glucosidase activity was observed. This activity was identified as lysosome alpha-(1-->4)-glucosidase. A single gel-filtration step resulted in a 700-fold purification of the enzyme. The same technique was also used to purify the acid alpha-glucosidase of human kidney. 2. The acid alpha-glucosidases of both tissues show very similar pH optima when tested with maltose or glycogen as substrate.

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Year:  1967        PMID： 6056633      PMCID： PMC1198270          DOI： 10.1042/bj1050035

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  LIVER ALPHA-GLUCOSIDASES.

Authors:  H N TORRES; J M OLAVARRIA
Journal:  J Biol Chem       Date:  1964-08       Impact factor: 5.157

2.  [THE DETERMINATION OF THE MOLECULAR WEIGHT OF ENZYMES BY DEXTRAN GEL FILTRATION].

Authors:  F AURICCHIO; C B BRUNI
Journal:  Biochem Z       Date:  1964-08-11

3.  [On the molecular weight of lactate dehydrogenase. Studies on the behavior in gel filtration].

Authors:  T WIELAND; P DUESBERG; H DETERMANN
Journal:  Biochem Z       Date:  1963

4.  Determination of maltase and isomaltase activities with a glucose-oxidase reagent.

Authors:  A DAHLQVIST
Journal:  Biochem J       Date:  1961-09       Impact factor: 3.857

5.  [Comparative studies of quantitative determination of proteins in cerebrospinal fluid and low-protein solutions].

Authors:  M EGGSTEIN; F H KREUTZ
Journal:  Klin Wochenschr       Date:  1955-10-01

6.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

7.  The subcellular distribution of enzymes in type II glycogenosis and the occurrence of an oligo-alpha-1,4-glucan glucohydrolase in human tissues.

Authors:  B I Brown; D H Brown
Journal:  Biochim Biophys Acta       Date:  1965-10-25

8.  Dextran-gel filtration of enzymes in the presence of their high-molecular-weight substrates.

Authors:  F Auricchio; C B Bruni
Journal:  Biochem J       Date:  1966-01       Impact factor: 3.857

9.  alpha-Glucosidase deficiency in generalized glycogenstorage disease (Pompe's disease).

Authors:  H G HERS
Journal:  Biochem J       Date:  1963-01       Impact factor: 3.857

10.  Estimation of the molecular weights of proteins by Sephadex gel-filtration.

Authors:  P Andrews
Journal:  Biochem J       Date:  1964-05       Impact factor: 3.766
  10 in total
  5 in total

Review 1.  Immunoglobulins and immunocytes.

Authors:  J J Cebra
Journal:  Bacteriol Rev       Date:  1969-06

2.  Type 3 a glycogenosis. A biochemical and ultrastructural study.

Authors:  F M Paluello; C B Bruni; H Spiele
Journal:  Virchows Arch Pathol Anat Physiol Klin Med       Date:  1967

3.  Further purification and characterization of the acid alpha-glucosidase.

Authors:  F Auricchio; C B Bruni; V Sica
Journal:  Biochem J       Date:  1968-06       Impact factor: 3.857

4.  Studies on mammalian glucoamylases with special reference to monkey intestinal glucoamylase.

Authors:  B Seetharam; N Swaminathan; A N Radhakrishnan
Journal:  Biochem J       Date:  1970-05       Impact factor: 3.857

5.  The substrate specificity of acid -glucosidase from rabbit muscle.

Authors:  T N Palmer
Journal:  Biochem J       Date:  1971-10       Impact factor: 3.857
  5 in total

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