Literature DB >> 605382

A procedure for the fusion of cells in suspension by means of polyethylene glycol.

A Hales.   

Abstract

The nuclei of chick and human fibroblasts are readily distinguishable in Leishman stained heterokaryons. The use of these two types of cell has allowed development of a method for fusion in suspension based on the use of polyethylene glycol (PEG) of MW 1000 in Dulbecco's medium containing 15% dimethylsulfoxide (DMSO).

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Year:  1977        PMID： 605382     DOI： 10.1007/bf01551817

Source DB:  PubMed          Journal:  Somatic Cell Genet        ISSN： 0098-0366


  19 in total

1.  Transfer of glucagon receptor from liver membranes to a foreign adenylate cyclase by a membrane fusion procedure.

Authors:  M Schramm
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

2.  Gene assignment by means of somatic cell hybrids: a new method for the analysis of data.

Authors:  H R Roth; G Dolf; G Stranzinger
Journal:  Theor Appl Genet       Date:  1987-05       Impact factor: 5.699

3.  Quiescent human diploid cells can inhibit entry into S phase in replicative nuclei in heterodikaryons.

Authors:  G H Stein; R M Yanishevsky
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

4.  Rat monoclonal antibodies which inhibit the in vitro multiplication of Plasmodium knowlesi.

Authors:  J A Deans; T Alderson; A W Thomas; G H Mitchell; E S Lennox; S Cohen
Journal:  Clin Exp Immunol       Date:  1982-08       Impact factor: 4.330

5.  Condensation of all human chromosomes in phase G2 and early mitosis can be drastically inhibited by 33258-Hoechst treatment.

Authors:  M Marcus; R Goitein; A Gropp
Journal:  Hum Genet       Date:  1979-09-02       Impact factor: 4.132

6.  Properties and fluctuations in vivo of rat liver antizyme inhibitor.

Authors:  Y Murakami; S Matsufuji; M Nishiyama; S Hayashi
Journal:  Biochem J       Date:  1989-05-01       Impact factor: 3.857

7.  Xeroderma pigmentosum patients from Germany: clinical symptoms and DNA repair characteristics.

Authors:  E Fischer; H W Thielmann; B Neundörfer; F J Rentsch; L Edler; E G Jung
Journal:  Arch Dermatol Res       Date:  1982       Impact factor: 3.017

8.  Variable X chromosome inactivation patterns in near-tetraploid murine EC x somatic cell hybrid cells differentiated in vitro.

Authors:  N Takagi
Journal:  Genetica       Date:  1993       Impact factor: 1.082

9.  Tumour localisation by human monoclonal antibodies.

Authors:  K Sikora; T Alderson; A Nethersell; H Smedley
Journal:  Med Oncol Tumor Pharmacother       Date:  1985

10.  Expression of macrophage functions in hybrids of a myeloma cell line with inflammatory macrophages: evidence for negative control mechanisms in the expression of macrophage functions.

Authors:  S Yamamoto; Y Higuchi
Journal:  Immunogenetics       Date:  1984       Impact factor: 2.846
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